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J. Biol. Chem., Vol. 282, Issue 6, 3881-3888, February 9, 2007

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Passive and Facilitated Transport in Nuclear Pore Complexes Is Largely Uncoupled^*

Bracha Naim, Vlad Brumfeld, Ruti Kapon, Vladimir Kiss, Reinat Nevo, and Ziv Reich¹

From the Departments of Biological Chemistry and Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel

Nuclear pore complexes provide the sole gateway for the exchange of material between nucleus and cytoplasm of interphase eukaryotic cells. They support two modes of transport: passive diffusion of ions, metabolites, and intermediate-sized macromolecules and facilitated, receptor-mediated translocation of proteins, RNA, and ribonucleoprotein complexes. It is generally assumed that both modes of transport occur through a single diffusion channel located within the central pore of the nuclear pore complex. To test this hypothesis, we studied the mutual effects between transporting molecules utilizing either the same or different modes of translocation. We find that the two modes of transport do not interfere with each other, but molecules utilizing a particular mode of transport do hinder motion of others utilizing the same pathway. We therefore conclude that the two modes of transport are largely segregated.

Received for publication, August 31, 2006 , and in revised form, November 21, 2006.

^* This work was supported by grants from the Human Frontier Science Program and from the Minerva Foundation with funding from the federal German ministry for education and research. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 972-8-934-2982; Fax: 972-8-934-6010; E-mail: ziv.reich{at}weizmann.ac.il.

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