HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 7, 4373-4381, February 16, 2007

This Article Full Text Full Text (PDF) Supplemental Data Supplemental Data All Versions of this Article: 282/7/4373    most recent M607145200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Larose, S. Articles by Elela, S. A. Search for Related Content PubMed PubMed Citation Articles by Larose, S. Articles by Elela, S. A. Social Bookmarking                      What's this?

RNase III-dependent Regulation of Yeast Telomerase^*

From the RNA Group, Département de Microbiologie et d’Infectiologie, Facultéde Médecine, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada

In bakers’ yeast, in vivo telomerase activity requires a ribonucleoprotein (RNP) complex with at least four associated proteins (Est2p, Est1p, Est3p, and Cdc13p) and one RNA species (Tlc1). The function of telomerase in maintaining chromosome ends, called telomeres, is tightly regulated and linked to the cell cycle. However, the mechanisms that regulate the expression of individual components of telomerase are poorly understood. Here we report that yeast RNase III (Rnt1p), a double-stranded RNA-specific endoribonuclease, regulates the expression of telomerase subunits and is required for maintaining normal telomere length. Deletion or inactivation of RNT1 induced the expression of Est1, Est2, Est3, and Tlc1 RNAs and increased telomerase activity, leading to elongation of telomeric repeat tracts. In silico analysis of the different RNAs coding for the telomerase subunits revealed a canonical Rnt1p cleavage site near the 3' end of Est1 mRNA. This predicted structure was cleaved by Rnt1p and its disruption abolished cleavage in vitro. Mutation of the Rnt1p cleavage signal in vivo impaired the cell cycle-dependent degradation of Est1 mRNA without affecting its steady-state level. These results reveal a new mechanism that influences telomeres length by controlling the expression of the telomerase subunits.

Received for publication, July 27, 2006 , and in revised form, December 6, 2006.

^* This work was supported by Grants MOP-67162 and MOP-74438 from the Canadian Institutes for Health Research. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Table S1 and Figs. S1–S2.

¹ Chercheur National of the Fonds de la Recherche en Santé du Québec.

² Chercheur-Boursier Senior scientist. To whom correspondence should be addressed: 3001 12^e Ave. Nord, Sherbrooke, Québec J1H 5N4, Canada. Tel.: 819-564-5275; Fax: 819-564-5392; E-mail: sherif.abou.elela{at}usherbrooke.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. D. Mozdy, E. R. Podell, and T. R. Cech Multiple Yeast Genes, Including Paf1 Complex Genes, Affect Telomere Length via Telomerase RNA Abundance Mol. Cell. Biol., June 15, 2008; 28(12): 4152 - 4161. [Abstract] [Full Text] [PDF]

				M. Catala, M. Tremblay, E. Samson, A. Conconi, and S. Abou Elela Deletion of Rnt1p Alters the Proportion of Open versus Closed rRNA Gene Repeats in Yeast Mol. Cell. Biol., January 15, 2008; 28(2): 619 - 629. [Abstract] [Full Text] [PDF]