HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 10, 6076-6084, March 7, 2008

This Article Full Text Full Text (PDF) All Versions of this Article: 283/10/6076    most recent M709189200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ueyama, M. Articles by Nishihara, S. Search for Related Content PubMed PubMed Citation Articles by Ueyama, M. Articles by Nishihara, S. Social Bookmarking                      What's this?

Functional Analysis of Proteoglycan Galactosyltransferase II RNA Interference Mutant Flies^*

Morio Ueyama, Hitoshi Takemae, Yoshiko Ohmae, Hideki Yoshida, Hidenao Toyoda^¶, Ryu Ueda^||, and Shoko Nishihara¹

From the Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577, the Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, the ^¶Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, and the ^||Invertebrate Genetics Laboratory, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 441-8540, Japan

Heparan sulfate proteoglycan plays an important role in developmental processes by modulating the distribution and stability of the morphogens Wingless, Hedgehog, and Decapentaplegic. Heparan and chondroitin sulfates share a common linkage tetrasaccharide structure, GlcAβ1,3Galβ1,3Galβ1,4Xylβ-O-Ser. In the present study, we identified Drosophila proteoglycan galactosyltransferase II (dβ3GalTII), determined its substrate specificity, and performed its functional analysis by using RNA interference (RNAi) mutant flies. The enzyme transferred a galactose to Galβ1,4Xyl-pMph, confirming that it is the Drosophila ortholog of human proteoglycan galactosyltransferase II. Real-time PCR analyses revealed that dβ3GalTII is expressed in various tissues and throughout development. The dβ3GalTII RNAi mutant flies showed decreased amounts of heparan sulfate proteoglycans. A genetic interaction of dβ3GalTII with Drosophila β1,4-galactoslyltransferase 7 (dβ4GalT7) or with six genes that encode enzymes contributing to the synthesis of glycosaminoglycans indicated that dβ3GalTII is involved in heparan sulfate synthesis for wing and eye development. Moreover, dβ3GalTII knock-down caused a decrease in extracellular Wingless in the wing imaginal disc of the third instar larvae. These results demonstrated that dβ3GalTII contributes to heparan sulfate proteoglycan synthesis in vitro and in vivo and also modulates Wingless distribution.

Received for publication, November 8, 2007 , and in revised form, December 23, 2007.

^* This work was supported by the Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (JST). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Laboratory of Cell Biology, Dept. of Bioinformatics, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577, Japan. Tel.: 81-426-91-8140; Fax: 81-426-91-8140; E-mail: shoko{at}t.soka.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. G. T. Hagen, L. Zhang, E Tian, and Y. Zhang Glycobiology on the fly: Developmental and mechanistic insights from Drosophila Glycobiology, February 1, 2009; 19(2): 102 - 111. [Abstract] [Full Text] [PDF]