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J. Biol. Chem., Vol. 283, Issue 10, 6261-6271, March 7, 2008

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TINY, a Dehydration-responsive Element (DRE)-binding Protein-like Transcription Factor Connecting the DRE- and Ethylene-responsive Element-mediated Signaling Pathways in Arabidopsis^*

Shan Sun, Jun-Ping Yu^¶, Feng Chen, Tong-Jin Zhao, Xiao-Hong Fang^¶, Yi-Qin Li, and Sen-Fang Sui¹

From the Department of Biological Sciences and Biotechnology and the State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084 and ^¶Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Dehydration-responsive element-binding proteins (DREBs) and ethylene-responsive element (ERE) binding factors are two major subfamilies of the AP2/ethylene-responsive element-binding protein family and play crucial roles in the regulation of abiotic- and biotic-stress responses, respectively. In the present work, we have reported a previously identified DREB-like factor, TINY, that was involved in both abiotic- and biotic-stress signaling pathways. TINY was capable of binding to both DRE and ERE with similar affinity and could activate the expression of reporter genes driven by either of these two elements in tobacco cells. The 15th amino acid in the APETALA2 (AP2)/ethylene-responsive element-binding factor domain was demonstrated to be essential for its specific binding to ERE, whereas the 14th and 19th amino acids were responsible for the binding to DRE. The expression of TINY was greatly activated by drought, cold, ethylene, and slightly by methyl jasmonate. Additionally, overexpression of TINY in Arabidopsis resulted in elevated expressions of both the DRE- and the ERE-containing genes. Moreover, the expression of DRE-regulated genes, such as COR6.6 and ERD10, was up-regulated upon ethylene treatment, and the expression of ERE-regulated genes, such as HLS1, was also increased by cold stress, when the expression of TINY was being induced. These results strongly suggested that TINY might play a role in the cross-talk between abiotic- and biotic-stress-responsive gene expressions by connecting the DRE- and ERE-mediated signaling pathways. The results herein might promote the understanding of the mechanisms of specific DNA recognition and gene expression regulation by DREBs.

Received for publication, August 15, 2007 , and in revised form, December 18, 2007.

^* This work was supported by the National Nature Science Foundation of China (Grants 30628007, 30330160, and 30670501) and the National Basic Research Program of China (Grant 2004CB720005). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 86-10-6278-4768; Fax: 86-10-6279-3367; E-mail: suisf{at}mail.tsinghua.edu.cn.

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