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J. Biol. Chem., Vol. 283, Issue 11, 7206-7218, March 14, 2008

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A Single Luminally Continuous Sarcoplasmic Reticulum with Apparently Separate Ca²⁺ Stores in Smooth Muscle^*

From the Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, John Arbuthnott Building, 27 Taylor Street, Glasgow G4 0NR, Scotland, United Kingdom

Whether or not the sarcoplasmic reticulum (SR) is a continuous, interconnected network surrounding a single lumen or comprises multiple, separate Ca²⁺ pools was investigated in voltage-clamped single smooth muscle cells using local photolysis of caged compounds and Ca²⁺ imaging. The entire SR could be depleted or refilled from one small site via either inositol 1,4,5-trisphosphate receptors (IP₃R) or ryanodine receptors (RyR) suggesting the SR is luminally continuous and that Ca²⁺ may diffuse freely throughout. Notwithstanding, regulation of the opening of RyR and IP₃R, by the [Ca²⁺] within the SR, may create several apparent SR elements with various receptor arrangements. IP₃R and RyR may appear to exist entirely on a single store, and there may seem to be additional SR elements that express either only RyR or only IP₃R. The various SR receptor arrangements and apparently separate Ca²⁺ storage elements exist in a single luminally continuous SR entity.

Received for publication, October 30, 2007 , and in revised form, December 14, 2007.

^* This work was supported by Wellcome Trust Grant 078054/Z/05/Z and British Heart Foundation Grant PG/06/016. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 44-141-548-4419; Fax: 44-141-552-2562; E-mail: john.mccarron{at}strath.ac.uk.

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