HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 12, 7346-7353, March 21, 2008

This Article Full Text Full Text (PDF) All Versions of this Article: 283/12/7346    most recent M708846200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pérez, J. M. Articles by Vásquez, C. C. Search for Related Content PubMed PubMed Citation Articles by Pérez, J. M. Articles by Vásquez, C. C. Social Bookmarking                      What's this?

Escherichia coli YqhD Exhibits Aldehyde Reductase Activity and Protects from the Harmful Effect of Lipid Peroxidation-derived Aldehydes^*

From the Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago 9170019, Chile

Evidence that Escherichia coli YqhD is involved in bacterial response to compounds that generate membrane lipid peroxidation is presented. Overexpression of yqhD results in increased resistance to the reactive oxygen species-generating compounds hydrogen peroxide, paraquat, chromate, and potassium tellurite. Increased tolerance was also observed for the lipid peroxidation-derived aldehydes butanaldehyde, propanaldehyde, acrolein, and malondialdehyde and the membrane-peroxidizing compound tert-butylhydroperoxide. Expression of yqhD was also associated with changes in the concentration of intracellular peroxides and cytoplasmic protein carbonyl content and with a reduction in intracellular acrolein levels. When compared with the wild type strain, an yqhD mutant exhibited a sensitive phenotype to all these compounds and also augmented levels of thiobarbituric acid-reactive substances, which may indicate an increased level of lipid peroxidation. Purified YqhD catalyzes the in vitro reduction of acetaldehyde, malondialdehyde, propanaldehyde, butanaldehyde, and acrolein in a NADPH-dependent reaction. Finally, yqhD transcription was induced in cells that had been exposed to conditions favoring lipid peroxidation. Taken together these results indicate that this enzyme may have a physiological function by protecting the cell against the toxic effect of aldehydes derived from lipid oxidation. We speculate that in Escherichia coli YqhD is part of a glutathione-independent, NADPH-dependent response mechanism to lipid peroxidation.

Received for publication, October 26, 2007 , and in revised form, January 16, 2008.

^* This work received financial support from Fondo Nacional de Ciencia y Tecnologia Grant 1060022 and by Dicyt (Universidad de Santiago de Chile) grants (to C. C. V.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Recipient of a doctoral fellowship from Mejoramiento de la Calidad y la Equidad de la Educacion Superior (MECESUP).

² Recipient of a doctoral fellowship from Consejo Nacional de Ciencia y Tecnologia.

³ To whom correspondence should be addressed: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile. Tel.: 56-2-681-0357; Fax: 56-2-681-2108; E-mail: cvasquez{at}usach.cl.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. N. Miller, L. R. Jarboe, L. P. Yomano, S. W. York, K. T. Shanmugam, and L. O. Ingram Silencing of NADPH-Dependent Oxidoreductase Genes (yqhD and dkgA) in Furfural-Resistant Ethanologenic Escherichia coli Appl. Envir. Microbiol., July 1, 2009; 75(13): 4315 - 4323. [Abstract] [Full Text] [PDF]

				I. L. Calderon, A. O. Elias, E. L. Fuentes, G. A. Pradenas, M. E. Castro, F. A. Arenas, J. M. Perez, and C. C. Vasquez Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases Microbiology, June 1, 2009; 155(6): 1840 - 1846. [Abstract] [Full Text] [PDF]