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J. Biol. Chem., Vol. 283, Issue 16, 10287-10296, April 18, 2008

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Mechanism and Regulation of the Two-component FMN-dependent Monooxygenase ActVA-ActVB from Streptomyces coelicolor^*

Julien Valton, Carole Mathevon^¶||, Marc Fontecave^¶||, Vincent Nivière^¶||1, and David P. Ballou²

From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-0606, the Laboratoire de Chimie et Biologie des Métaux, CEA, DSV, iRTSV,17 Avenue des Martyrs, Grenoble F-38054, France, the ^¶CNRS, UMR 5249, Grenoble F-38054, France, and the ^||Université Joseph Fourier, Grenoble F-38000, France

The ActVA-ActVB system from Streptomyces coelicolor is a two-component flavin-dependent monooxygenase involved in the antibiotic actinorhodin biosynthesis. ActVB is a NADH:flavin oxidoreductase that provides a reduced FMN to ActVA, the monooxygenase that catalyzes the hydroxylation of dihydrokalafungin, the precursor of actinorhodin. In this work, using stopped-flow spectrophotometry, we investigated the mechanism of hydroxylation of dihydrokalafungin catalyzed by ActVA and that of the reduced FMN transfer from ActVB to ActVA. Our results show that the hydroxylation mechanism proceeds with the participation of two different reaction intermediates in ActVA active site. First, a C(4a)-FMN-hydroperoxide species is formed after binding of reduced FMN to the monooxygenase and reaction with O₂. This intermediate hydroxylates the substrate and is transformed to a second reaction intermediate, a C(4a)-FMN-hydroxy species. In addition, we demonstrate that reduced FMN can be transferred efficiently from the reductase to the monooxygenase without involving any protein·protein complexes. The rate of transfer of reduced FMN from ActVB to ActVA was found to be controlled by the release of NAD⁺ from ActVB and was strongly affected by NAD⁺ concentration, with an IC₅₀ of 40 µM. This control of reduced FMN transfer by NAD⁺ was associated with the formation of a strong charge·transfer complex between NAD⁺ and reduced FMN in the active site of ActVB. These results suggest that, in Streptomyces coelicolor, the reductase component ActVB can act as a regulatory component of the monooxygenase activity by controlling the transfer of reduced FMN to the monooxygenase.

Received for publication, November 28, 2007 , and in revised form, January 30, 2008.

^* This work was supported by National Institutes of Health Grant GM64711 (to D. P. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence may be addressed. Tel.: 33-4-38-78-91-09; Fax: 33-4-38-78-91-24; E-mail: vniviere{at}cea.fr. ² To whom correspondence may be addressed. Tel.: 734-764-9582; Fax: 734-764-3509; E-mail: dballou{at}umich.edu.

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