|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
J. Biol. Chem., Vol. 283, Issue 16, 11064-11071, April 18, 2008
Prostaglandin E2 Attenuates Preoptic Expression of GABAA Receptors via EP3 Receptors* 1 ¶![]() ![]() 3
From the
Prostaglandin E2 (PGE2) has been shown to produce fever by acting on EP3 receptors within the preoptic area of the brain. However, there is little information about the molecular events downstream of EP3 activation in preoptic neurons. As a first step toward this issue, we examined PGE2-induced gene expression changes at single-cell resolution in preoptic neurons expressing EP3. Brain sections of the preoptic area from PGE2- or saline-injected rats were stained with an anti-EP3 antibody, and the cell bodies of EP3-positive neurons were dissected and subjected to RNA amplification procedures. Microarray analysis of the amplified products demonstrated the possibility that gene expression of
Received for publication, February 20, 2008 * This work was supported in part by a grant from the Sankyo Foundation of Life Science and by grants-in-aid for scientific research (B) and on priority areas (Applied Genomics, Mechanisms of Sex Differentiation, and Molecular Brain Science) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Ministry of Health and Labor of Japan, respectively. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 Supported by a research fellowship from the Japan Society for the Promotion of Science for Young Scientists. 2 Supported by a fellowship from the Japan Society for the Promotion of Science. 3 To whom correspondence should be addressed: Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Fax: 81-75-753-4557; E-mail: ysugimot{at}pharm.kyoto-u.ac.jp.
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||