HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 17, 11364-11373, April 25, 2008

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 283/17/11364    most recent M710106200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vogel, J. T. Articles by Klee, H. J. Search for Related Content PubMed PubMed Citation Articles by Vogel, J. T. Articles by Klee, H. J. Social Bookmarking                      What's this?

The Carotenoid Cleavage Dioxygenase 1 Enzyme Has Broad Substrate Specificity, Cleaving Multiple Carotenoids at Two Different Bond Positions^*

From the Horticultural Sciences Department and the Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611

In many organisms, various enzymes mediate site-specific carotenoid cleavage to generate biologically active apocarotenoids. These carotenoid-derived products include provitamin A, hormones, and flavor and fragrance molecules. In plants, the CCD1 enzyme cleaves carotenoids at 9,10 (9',10') bonds to generate multiple apocarotenoid products. Here we systematically analyzed volatile apocarotenoids generated by maize CCD1 (ZmCCD1) from multiple carotenoid substrates. ZmCCD1 did not cleave geranylgeranyl diphosphate or phytoene but did cleave other linear and cyclic carotenoids, producing volatiles derived from 9,10 (9',10') bond cleavage. Additionally the Arabidopsis, maize, and tomato CCD1 enzymes all cleaved lycopene to generate 6-methyl-5-hepten-2-one. 6-Methyl-5-hepten-2-one, an important flavor volatile in tomato, was produced by cleavage of the 5,6 or 5',6' bond positions of lycopene but not geranylgeranyl diphosphate, -carotene, or phytoene. In vitro, ZmCCD1 cleaved linear and cyclic carotenoids with equal efficiency. Based on the pattern of apocarotenoid volatiles produced, we propose that CCD1 recognizes its cleavage site based on the saturation status between carbons 7 and 8 (7' and 8') and carbons 11 and 12 (11' and 12') as well as the methyl groups on carbons 5, 9, and 13 (5', 9', and 13').

Received for publication, December 11, 2007 , and in revised form, January 25, 2008.

^* This work was supported by National Science Foundation Grant IOB-0446040 (to H. J. K. and D. R. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1 and 2.

¹ To whom correspondence should be addressed: Horticultural Sciences Dept., Plant Molecular and Cellular Biology Program, University of Florida, P. O. Box 110690, Gainesville, FL 32611. Tel.: 352-392-8249; Fax: 352-846-2063; E-mail: hjklee{at}ifas.ufl.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Mathieu, V. Dal Cin, Z. Fei, H. Li, P. Bliss, M. G. Taylor, H. J. Klee, and D. M. Tieman Flavour compounds in tomato fruits: identification of loci and potential pathways affecting volatile composition J. Exp. Bot., December 16, 2008; (2008) ern294v1. [Abstract] [Full Text] [PDF]

				B. L. Lindshield, J. L. King, A. Wyss, R. Goralczyk, C.-H. Lu, N. A. Ford, and J. W. Erdman Jr Lycopene Biodistribution Is Altered in 15,15'-Carotenoid Monooxygenase Knockout Mice J. Nutr., December 1, 2008; 138(12): 2367 - 2371. [Abstract] [Full Text] [PDF]

				D. S. Floss, W. Schliemann, J. Schmidt, D. Strack, and M. H. Walter RNA Interference-Mediated Repression of MtCCD1 in Mycorrhizal Roots of Medicago truncatula Causes Accumulation of C27 Apocarotenoids, Shedding Light on the Functional Role of CCD1 Plant Physiology, November 1, 2008; 148(3): 1267 - 1282. [Abstract] [Full Text] [PDF]

				A. Rubio, J. L. Rambla, M. Santaella, M. D. Gomez, D. Orzaez, A. Granell, and L. Gomez-Gomez Cytosolic and Plastoglobule-targeted Carotenoid Dioxygenases from Crocus sativus Are Both Involved in {beta}-Ionone Release J. Biol. Chem., September 5, 2008; 283(36): 24816 - 24825. [Abstract] [Full Text] [PDF]