HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 19, 13205-13215, May 9, 2008

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 283/19/13205    most recent M707629200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Y. Articles by Lee, C. G. L. Search for Related Content PubMed PubMed Citation Articles by Wang, Y. Articles by Lee, C. G. L. Social Bookmarking                      What's this?

Profiling MicroRNA Expression in Hepatocellular Carcinoma Reveals MicroRNA-224 Up-regulation and Apoptosis Inhibitor-5 as a MicroRNA-224-specific Target^*

Yu Wang¹, Alvin T. C. Lee¹, Joel Z. I. Ma, Jingbo Wang, Jianwei Ren, Yuchen Yang^¶2, Erwin Tantoso^¶, Kuo-Bin Li^¶3, London L. P. J Ooi^||**, Patrick Tan, and Caroline G. L. Lee⁴

From the Department of Biochemistry, National University of Singapore, Singapore 119077, Singapore, the Division of Medical Sciences and ^||Department of Surgical Oncology, National Cancer Centre, Singapore 169610, Singapore, the ^¶Bioinformatics Institute, Singapore 138671, Singapore, the ^**Department of Surgery, Singapore General Hospital, Singapore 169608, and the Duke-NUS Graduate Medical School, Singapore 169547, Singapore

Like other cancers, aberrant gene regulation features significantly in hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) were recently found to regulate gene expression at the post-transcriptional/translational levels. The expression profiles of 157 miRNAs were examined in 19 HCC patients, and 19 up-regulated and 3 down-regulated miRNAs were found to be associated with HCC. Putative gene targets of these 22 miRNAs were predicted in silico and were significantly enriched in 34 biological pathways, most of which are frequently dysregulated during carcinogenesis. Further characterization of microRNA-224 (miR-224), the most significantly up-regulated miRNA in HCC patients, revealed that miR-224 increases apoptotic cell death as well as proliferation and targets apoptosis inhibitor-5 (API-5) to inhibit API-5 transcript expression. Significantly, miR-224 expression was found to be inversely correlated with API-5 expression in HCC patients (p < 0.05). Hence, our findings define a true in vivo target of miR-224 and reaffirm the important role of miRNAs in the dysregulation of cellular processes that may ultimately lead to tumorigenesis.

Received for publication, September 11, 2007 , and in revised form, February 5, 2008.

^* This work was supported by a National Medical Research Council of Singapore block grant through the National Cancer Centre (to C. G. L.), an individual grant from the National Medical Research Council, and a grant from the BioMedical Research Council (to C. G. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1–S3 and Table S1.

¹ These authors contributed equally to this work.

² Present address: Institute of Molecular and Cell Biology, Singapore.

³ Present address: Bioinformatics Center, National Yang-Ming University, Taipei, 112, Taiwan.

⁴ To whom correspondence should be addressed: Division of Medical Sciences, National Cancer Center, Level 6, Lab 5, 11 Hospital Dr., Singapore 169610, Singapore. Tel.: 65-6436-8353; Fax: 65-6372-0161; E-mail: bchleec{at}nus.edu.sg.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				X. LIU, J. YU, L. JIANG, A. WANG, F. SHI, H. YE, and X. ZHOU MicroRNA-222 Regulates Cell Invasion by Targeting Matrix Metalloproteinase 1 (MMP1) and Manganese Superoxide Dismutase 2 (SOD2) in Tongue Squamous Cell Carcinoma Cell Lines Cancer Genomics Proteomics, May 1, 2009; 6(3): 131 - 139. [Abstract] [Full Text] [PDF]

				H. Su, J.-R. Yang, T. Xu, J. Huang, L. Xu, Y. Yuan, and S.-M. Zhuang MicroRNA-101, Down-regulated in Hepatocellular Carcinoma, Promotes Apoptosis and Suppresses Tumorigenicity Cancer Res., February 1, 2009; 69(3): 1135 - 1142. [Abstract] [Full Text] [PDF]

				Y. Pei, T. Zhang, V. Renault, and X. Zhang An overview of hepatocellular carcinoma study by omics-based methods Acta Biochim Biophys Sin, January 1, 2009; 41(1): 1 - 15. [Abstract] [Full Text] [PDF]