HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 2, 940-950, January 11, 2008

This Article Full Text Full Text (PDF) All Versions of this Article: 283/2/940    most recent M706647200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kuraoka, I. Articles by Tanaka, K. Search for Related Content PubMed PubMed Citation Articles by Kuraoka, I. Articles by Tanaka, K. Social Bookmarking                      What's this?

Isolation of XAB2 Complex Involved in Pre-mRNA Splicing, Transcription, and Transcription-coupled Repair^*

Isao Kuraoka¹, Shinsuke Ito, Tadashi Wada^¶, Mika Hayashida, Lily Lee², Masafumi Saijo, Yoshimichi Nakatsu³, Megumi Matsumoto^||, Tsukasa Matsunaga^||, Hiroshi Handa^¶, Jun Qin^**, Yoshihiro Nakatani, and Kiyoji Tanaka⁴

From the Laboratories for Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University and Solution-oriented Research for Science and Technology, Japan Science and Technology Agency, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan, ^||Laboratory of Human Molecular Genetics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan, ^¶Faculty of Bioscience and Biotechnology and Frontier Collaborative Research Center, Tokyo Institute of Technology, Yokohama 226-8501, Japan, the ^**Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, and the Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115

Nucleotide excision repair is a versatile repair pathway that counteracts the deleterious effects of various DNA lesions. In nucleotide excision repair, there is a transcription-coupled repair (TCR) pathway that focuses on DNA damage that blocks RNA polymerase IIo in transcription elongation. XAB2 (XPA-binding protein 2), containing tetratricopeptide repeats, has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A protein (XPA). Moreover, XAB2 has been shown to interact with Cockayne syndrome group A and B proteins (CSA and CSB) and RNA polymerase II, as well as XPA, and is involved in TCR and transcription. Here we purified XAB2 as a multimeric protein complex consisting of hAquarius, XAB2, hPRP19, CCDC16, hISY1, and PPIE, which are involved in pre-mRNA splicing. Knockdown of XAB2 with small interfering RNA in HeLa cells resulted in a hypersensitivity to killing by UV light and a decreased recovery of RNA synthesis after UV irradiation and regular RNA synthesis. Enhanced interaction of XAB2 with RNA polymerase IIo or XPA was observed in cells treated with DNA-damaging agents, indicating DNA damage-responsive activity of the XAB2 complex. These results indicated that the XAB2 complex is a multifunctional factor involved in pre-mRNA splicing, transcription, and TCR.

Received for publication, August 10, 2007 , and in revised form, October 29, 2007.

^* This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Solution-oriented Research for Science and Technology of Japan Science and Technology Corp. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

² Recipient of an Advanced Biotechnology Scholarship from Ishihara Sangyo Kaisha, Ltd., in collaboration with the Singapore Economic Development Board.

³ Present address: Dept. of Medical Biophysics and Radiation Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812-8582, Japan.

¹ To whom correspondence may be addressed. Tel.: 81-92-541-3231; Fax: 81-92-542-8534; E-mail: ikuraoka{at}nk-cc.go.jp. ⁴ To whom correspondence may be addressed. Tel.: 81-6-6879-7971; Fax: 81-6-6877-9136; E-mail: ktanaka{at}fbs.osaka-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?