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J. Biol. Chem., Vol. 283, Issue 20, 13725-13735, May 16, 2008

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An Intramolecular Route for Coupling ATPase Activity in AAA⁺ Proteins for Transcription Activation^*

From the Division of Biology, Sir Alexander Fleming Building, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom

AAA⁺ proteins (ATPases associated with various cellular activities) contribute to many cellular processes and typically function as higher order oligomers permitting the coordination of nucleotide hydrolysis for functional output, which leads to substrate remodeling. The precise mechanisms that enable the relay of nucleotide hydrolysis to their specific functional outputs are largely unknown. Here we use PspF, a specialized AAA⁺ protein required for enhancer-dependent transcription activation in Escherichia coli, as a model system to address this question. We demonstrate that a conserved asparagine is involved in internal organization of the oligomeric ring, regulation of ATPase activity by "trans" factors, and optimizing substrate remodeling. We provide evidence that the spatial relationship between the asparagine residue and the Walker B motif is one key element in the conformational signaling pathway that leads to substrate remodeling. Such functional organization most likely applies to other AAA⁺ proteins, including Ltag (simian virus 40), Rep40 (Adeno-associated virus-2), and p97 (Mus musculus) in which the asparagine to Walker B motif relationship is conserved.

Received for publication, January 30, 2008 , and in revised form, March 4, 2008.

^* This work was supported in part by the Leverhulme Trust and the Wellcome Trust for project support for cross-linking experiments. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1–S4.

¹ Recipient of European Molecular Biology Organization Fellowship ALTF 387-2005.

² To whom correspondence should be addressed. Tel.: 44-207-594-5442; Fax: 44-207-594-5419; E-mail: m.buck{at}imperial.ac.uk.