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J. Biol. Chem., Vol. 283, Issue 26, 17846-17854, June 27, 2008

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O-Glucosylation and O-Fucosylation Occur Together in Close Proximity on the First Epidermal Growth Factor Repeat of AMACO (VWA2 Protein)^*

Jan M. Gebauer¹, Stefan Müller, Franz-Georg Hanisch, Mats Paulsson, and Raimund Wagener²

From the Center for Biochemistry and Center for Molecular Medicine, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany

AMACO (VWA2 protein) is an extracellular matrix protein of unknown function associated with certain basement membranes in skin, lung, and kidney. AMACO is a member of the von Willebrand factor A-like (VWA) domain containing protein superfamily and in addition to three VWA domains it also contains two epidermal growth factor-like domains. One of these contains the rare, overlapping consensus sequences for both O-glucosylation and O-fucosylation. In earlier studies of other proteins the attachment of either core glucose and fucose moieties or of the respective elongated glycans starting with these monosaccharides has been described. By a detailed mass spectrometric analysis we show that both elongated O-glucosylated (Xyl1–3Xyl1–3Glc) and elongated O-fucosylated glycan chains (NeuAc2–3Gal1–4GlcNAc1–3Fuc) can be attached to AMACO in close proximity on the same epidermal growth factor-like domain. It has been reported that the lack of O-fucosylation can markedly decrease secretion of proteins. However, the secretion of AMACO is not significantly affected when the glycosylation sites are mutated. The number of extracellular matrix proteins carrying the overlapping consensus sequence is very limited and it could be that these modifications have a new, yet unknown function.

Received for publication, June 12, 2007 , and in revised form, April 3, 2008.

^* This work was supported by Deutsche Forschungsgemeinschaft Grants WA1338/2-3, WA1338/2-4, and WA1338/2-6 and the PRO INNO II Program of the German Federal Ministry of Economics and Technology Grant KF0055203UL6. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. S1.

¹ Member of the International Graduate School in Genetics and Functional Genomics at the University of Cologne.

² To whom correspondence should be addressed. Tel.: 49-221-478-6990; Fax: 49-221-478-6977; E-mail: raimund.wagener{at}uni-koeln.de.

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