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J. Biol. Chem., Vol. 283, Issue 26, 17881-17890, June 27, 2008

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Biogenesis of MalF and the MalFGK₂ Maltose Transport Complex in Escherichia coli Requires YidC^*

Samuel Wagner¹, Ovidio Pop¹, Gert-Jan Haan¹², Louise Baars, Gregory Koningstein, Mirjam M. Klepsch, Pierre Genevaux^¶, Joen Luirink³, and Jan-Willem de Gier⁴

From the Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm SE-106 91, Sweden, the Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, Amsterdam 1081 HV, The Netherlands, and the ^¶Laboratoire de Microbiologie et Génétique Moléculaires, Institut de Biologie Cellulaire et de Génétique, CNRS, Université Paul-Sabatier, 118 Route de Narbonne, Toulouse 31062, Cedex 09, France

The polytopic inner membrane protein MalF is a constituent of the MalFGK₂ maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK₂ maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.

Received for publication, February 25, 2008 , and in revised form, April 21, 2008.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ These authors contributed equally to this work.

² Supported by EU-network Grant QLK-C-T2000-00082. Centocor B.V., Einsteinweg 92, Leiden 2333 CD, The Netherlands.

³ To whom correspondence may be addressed. Tel.: 31-20-598-7175; Fax: 31-20-598-6979; E-mail: joen.luirink{at}falw.vu.nl. ⁴To whom correspondence may be addressed. Tel.: 46-8-162-420; Fax: 46-8-153-679; E-mail: degier{at}dbb.su.se.

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