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J. Biol. Chem., Vol. 283, Issue 31, 21469-21477, August 1, 2008

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The PHD Domain of Plant PIAS Proteins Mediates Sumoylation of Bromodomain GTE Proteins^*

Mario Garcia-Dominguez¹, Rosana March-Diaz, and Jose C. Reyes

From the Centro Andaluz de Biología Molecular y Medicina Regenerativa and Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, 41092 Seville, Spain

Covalent attachment of small ubiquitin-like modifier (SUMO) to proteins regulates multiple processes in the eukaryotic cell. In numerous cases sumoylation is facilitated by protein inhibitor of activated STAT (PIAS) proteins, characterized by the presence of a SP-RING domain related to the RING finger of many ubiquitin E3 ligases. The importance of SP-RING relies on its capacity to bind the E2 enzyme of the pathway. Additional domains may participate in SUMO ligase function and target selection. We have studied the Arabidopsis SUMO ligase AtSIZ1, belonging to the PIAS family, and describe self-sumoylation and AtSIZ1-mediated sumoylation of the E2 enzyme AtSCE1 and GTE3, a bromodomain protein interacting with AtSIZ1. Modification of GTE3 modulates its capacity to bind acetyl-histone H3 in vitro. Interestingly, AtSIZ1, as other plant PIAS proteins, also includes a PHD domain. We found that the PHD domain binds AtSCE1 and contributes to the SUMO ligase function, being partially and absolutely required for AtSCE1 and GTE3 sumoylation, respectively. Based on the capacity of AtSCE1 and GTE3 to associate with both the PHD and SP-RING domains, we propose a model of interactions to explain AtSIZ1-mediated sumoylation of GTE3 and ligase function of the PHD domain.

Received for publication, October 2, 2007 , and in revised form, March 3, 2008.

^* This work was funded by Ministerio de Educacion y Ciencia Grants BFU2006-14977 (to M. G.-D.) and BFU2005-01047 (to J. C. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: CABIMER, Av. Americo Vespucio, 41092 Seville, Spain. Tel.: 34-954-46-8061; Fax: 34-954-461664; E-mail: mario.garcia{at}cabimer.es.

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