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J. Biol. Chem., Vol. 283, Issue 35, 24185-24193, August 29, 2008

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Physical and Functional Interactions between Uracil-DNA Glycosylase and Proliferating Cell Nuclear Antigen from the Euryarchaeon Pyrococcus furiosus^*

Shinichi Kiyonari, Maiko Uchimura, Tsuyoshi Shirai^¶||, and Yoshizumi Ishino¹

From the Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, and BIRD-Japan Science and Technology Agency, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan and the ^¶Department of Bioscience, Nagahama Institute of Bio-Science and Technology and ^||BIRD-Japan Science and Technology Agency, 1266 Tamura, Nagahama, Shiga 526-0829, Japan

Uracil-DNA glycosylase (UDG) is an important repair enzyme in all organisms to remove uracil bases from DNA. Recent biochemical studies have revealed that human nuclear UDG (UNG2) forms a multiprotein complex in replication foci and initiates the base excision repair pathway by interacting with proliferating cell nuclear antigen (PCNA). Here, we show the physical and functional interactions between UDG and PCNA from the hyperthermophilic euryarchaeon, Pyrococcus furiosus. The physical interaction between the two proteins was identified by a surface plasmon resonance analysis. Furthermore, the uracil glycosylase activity of P. furiosus UDG is stimulated by P. furiosus PCNA (PfuPCNA) in vitro. This stimulatory effect was observed only when wild type PfuPCNA, but not a monomeric PCNA mutant, was present in the reaction. Mutational analyses revealed that our predicted PCNA-binding region (AKTLF) in P. furiosus UDG is actually important for the interaction with PfuPCNA. This is the first report describing the functional interaction between archaeal UDG and PCNA.

Received for publication, April 14, 2008 , and in revised form, June 10, 2008.

^* This work was supported in part by grants-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (to Y. I.). This work was also supported by the Hou-ansha Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1-S3.

¹ Supported by a research grant from the Human Frontier Science Program. To whom correspondence should be addressed: Dept. of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka-shi, Fukuoka 812-8581, Japan. Tel.: 81-92-642-4217; Fax: 81-92-642-3051; E-mail: ishino{at}agr.kyushu-u.ac.jp.

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