HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 46, 31511-31521, November 14, 2008

This Article Full Text Full Text (PDF) All Versions of this Article: 283/46/31511    most recent M804646200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, S.-s. Articles by Chen, T. Search for Related Content PubMed PubMed Citation Articles by Zhang, S.-s. Articles by Chen, T. Social Bookmarking                      What's this?

Plasminogen Activator Pla of Yersinia pestis Utilizes Murine DEC-205 (CD205) as a Receptor to Promote Dissemination^*

Shu-sheng Zhang, Chae Gyu Park¹, Pei Zhang, Sara Schesser Bartra^¶, Gregory V. Plano^¶, John D. Klena^||, Mikael Skurnik^**, B. Joseph Hinnebusch, and Tie Chen²

From the Department of Biomedical Sciences, College of Medicine-Rockford, University of Illinois at Chicago, Rockford, Illinois 61107, Laboratory of Cellular Physiology and Immunology, Chris Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, New York 10065, ^¶Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, Florida 33101, ^||School of Biological Sciences, University of Canterbury, Christchurch, New Zealand, ^**Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory Diagnostics, Helsinki 00014, Finland, Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, National Institutes of Health, Hamilton, Minnesota 59840, and Department of Molecular Biology, Umeå University, SE-901 87 Umeå, Sweden

Yersinia pestis, a Gram-negative bacterium that causes bubonic and pneumonic plague, is able to rapidly disseminate to other parts of its mammalian hosts. Y. pestis expresses plasminogen activator (PLA) on its surface, which has been suggested to play a role in bacterial dissemination. It has been speculated that Y. pestis hijacks antigen-presenting cells, such as macrophages (Ms) and dendritic cells, to be delivered to lymph nodes to initiate dissemination and infection. Both alveolar Ms and pulmonary dendritic cells express a C-type lectin receptor, DEC-205 (CD205), which mediates antigen uptake and presentation. However, no ligand has been identified for DEC-205. In this study, we show that the invasion of alveolar Msby Y. pestis depends both in vitro and in vivo on the expression of PLA. DEC-205-expressing Ms and transfectants, but not their negative counterparts, phagocytosed PLA-expressing Y. pestis and Escherichia coli K12 more efficiently than PLA-negative controls. The interactions between PLA-expressing bacteria and DEC-205-expressing transfectants or alveolar Ms could be inhibited by an anti-DEC-205 antibody. Importantly, the blockage of the PLA-DEC-205 interaction reduced the dissemination of Y. pestis in mice. In conclusion, murine DEC-205 is a receptor for PLA of Y. pestis, and this host-pathogen interaction appears to play a key role in promoting bacterial dissemination.

Received for publication, June 18, 2008 , and in revised form, July 14, 2008.

^* This work was supported, in whole or in part, by National Institutes of Health grants (USPHS). This work was also supported in part by a grant from the University of Illinois, College of Medicine, Rockford (to T. C.), and work in the Skurnik laboratory was supported by Academy of Finland Grant 114075. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Supported by National Institutes of Health Grant AI 057158 (Northeast Biodefense Center).

² To whom correspondence should be addressed: Dept. of Biomedical Sciences, College of Medicine-Rockford, University of Illinois at Chicago, 1601 Parkview Ave., Rockford, IL 61107. Tel.: 815-395-5893; Fax: 815-395-5666; E-mail: tiechen{at}uic.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Haiko, M. Suomalainen, T. Ojala, K. Lahteenmaki, and T. K. Korhonen Invited review: Breaking barriers -- attack on innate immune defences by omptin surface proteases of enterobacterial pathogens Innate Immunity, April 1, 2009; 15(2): 67 - 80. [Abstract] [PDF]