HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 5, 2644-2653, February 1, 2008

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 283/5/2644    most recent M707885200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Goldfeder, M. B. Articles by Oliveira, C. C. Search for Related Content PubMed PubMed Citation Articles by Goldfeder, M. B. Articles by Oliveira, C. C. Social Bookmarking                      What's this?

Cwc24p, a Novel Saccharomyces cerevisiae Nuclear Ring Finger Protein, Affects Pre-snoRNA U3 Splicing^*

From the Department of Biochemistry, Chemistry Institute, University of São Paulo, 748 Av. Prof. Lineu Prestes, São Paulo, SP, 05508-900, Brazil

U3 snoRNA is transcribed from two intron-containing genes in yeast, snR17A and snR17B. Although the assembly of the U3 snoRNP has not been precisely determined, at least some of the core box C/D proteins are known to bind pre-U3 co-transcriptionally, thereby affecting splicing and 3'-end processing of this snoRNA. We identified the interaction between the box C/D assembly factor Nop17p and Cwc24p, a novel yeast RING finger protein that had been previously isolated in a complex with the splicing factor Cef1p. Here we show that, consistent with the protein interaction data, Cwc24p localizes to the cell nucleus, and its depletion leads to the accumulation of both U3 pre-snoRNAs. U3 snoRNA is involved in the early cleavages of 35 S pre-rRNA, and the defective splicing of pre-U3 detected in cells depleted of Cwc24p causes the accumulation of the 35 S precursor rRNA. These results led us to the conclusion that Cwc24p is involved in pre-U3 snoRNA splicing, indirectly affecting pre-rRNA processing.

Received for publication, September 20, 2007 , and in revised form, October 31, 2007.

^* This work 7888888/^* was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo Grant 05/56493-9 (to C. C. O.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1–S4.

¹ Recipient of a Coordenação de Aperfeiçoamento de Pessoal de Nível Superior fellowship.

² To whom correspondence should be addressed. Tel.: 55-11-3091-3810 (ext. 208); Fax: 55-11-3815-5579; E-mail: ccoliv{at}iq.usp.br.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?