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J. Biol. Chem., Vol. 283, Issue 5, 2716-2723, February 1, 2008

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A Topological Model of the Baseplate of Lactococcal Phage Tuc2009^*

Giuliano Sciara, Stéphanie Blangy, Marina Siponen, Stephen Mc Grath, Douwe van Sinderen^¶, Mariella Tegoni, Christian Cambillau¹, and Valérie Campanacci²

From the Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and Universités Aix-Marseille I & II, 13288 Marseille, France, the Department of Microbiology, National University of Ireland, Cork, Ireland, and the ^¶Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland

Phages infecting Lactococcus lactis, a Gram-positive bacterium, are a recurrent problem in the dairy industry. Despite their economical importance, the knowledge on these phages, belonging mostly to Siphoviridae, lags behind that accumulated for members of Myoviridae. The three-dimensional structures of the receptor-binding proteins (RBP) of three lactococcal phages have been determined recently, illustrating their modular assembly and assigning the nature of their bacterial receptor. These RBPs are attached to the baseplate, a large phage organelle, located at the tip of the tail. Tuc2009 baseplate is formed by the products of 6 open read frames, including the RBP. Because phage binding to its receptor induces DNA release, it has been postulated that the baseplate might be the trigger for DNA injection. We embarked on a structural study of the lactococcal phages baseplate, ultimately to gain insight into the triggering mechanism following receptor binding. Structural features of the Tuc2009 baseplate were established using size exclusion chromatography coupled to on-line UV-visible absorbance, light scattering, and refractive index detection (MALS/UV/RI). Combining the results of this approach with literature data led us to propose a "low resolution" model of Tuc2009 baseplate. This model will serve as a knowledge base to submit relevant complexes to crystallization trials.

Received for publication, September 10, 2007 , and in revised form, November 7, 2007.

^* This work was supported by the Marseille-Nice Génopole, the CNRS, and through Science Foundation Ireland Investigator Grant 02/IN.1/B198 (to D. v. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence may be addressed: AFMB, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France. E-mail: cambillau{at}afmb.univ-mrs.fr. ² To whom correspondence may be addressed: AFMB, 163 Av. de Luminy Case 932, 13288 Marseille Cedex 09, France. Fax: 33-491-266-720; E-mail: valerie.campanacci{at}afmb.univ-mrs.fr.

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