HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 6, 3264-3271, February 8, 2008

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 283/6/3264    most recent M706419200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Desmots, F. Articles by McKinnon, P. J. Search for Related Content PubMed PubMed Citation Articles by Desmots, F. Articles by McKinnon, P. J. Social Bookmarking                      What's this?

Scythe Regulates Apoptosis-inducing Factor Stability during Endoplasmic Reticulum Stress-induced Apoptosis^*

Fabienne Desmots¹, Helen R. Russell, Denis Michel, and Peter J. McKinnon

From the Department of Genetics and Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105 and the UMR 6026, CNRS, Université de Rennes 1, 35042 Rennes Cedex, France

Scythe (BAT3; HLA-B associated transcript 3, Bag 6) is a protein that has been implicated in apoptosis because it can modulate the Drosophila melanogaster apoptotic regulator, Reaper. Mice lacking Scythe show pronounced defects in organogenesis and in the regulation of apoptosis and proliferation during mammalian development. However, the biochemical pathways important for Scythe function are unknown. We report here multiple levels of interaction between Scythe and the apoptogenic mitochondrial intermembrane protein AIF (apoptosis-inducing factor). Scythe physically interacts with AIF and regulates its stability. AIF stability is markedly reduced in Scythe^-/- cells, which are more resistant to endoplasmic reticulum stress induced by thapsigargin. Reintroduction of Scythe or overexpression of AIF in Scythe^-/- cells restores their sensitivity to apoptosis. Together, these data implicate Scythe as a regulator of AIF.

Received for publication, August 3, 2007 , and in revised form, November 26, 2007.

^* This work was supported by the National Institutes of Health and the American Lebanese and Syrian Associated Charities (ALSAC) of St. Jude Children's Research Hospital and the "Association pour la Recherche sur le Cancer". The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1–S3.

¹ To whom correspondence should be addressed. Tel.: 33-02-23-23-50-93; Fax: 33-02-23-23-50-52; E-mail: fabienne.desmots{at}univ-rennes1.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. H. Kwak, S. I. Kim, J. K. Kim, and M. E. Choi BAT3 Interacts with Transforming Growth Factor-{beta} (TGF-{beta}) Receptors and Enhances TGF-{beta}1-induced Type I Collagen Expression in Mesangial Cells J. Biol. Chem., July 11, 2008; 283(28): 19816 - 19825. [Abstract] [Full Text] [PDF]