HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 283, Issue 8, 4578-4587, February 22, 2008

This Article Full Text Full Text (PDF) All Versions of this Article: 283/8/4578    most recent M703456200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sammarco, M. C. Articles by Grabczyk, E. Search for Related Content PubMed PubMed Citation Articles by Sammarco, M. C. Articles by Grabczyk, E. Social Bookmarking                      What's this?

Ferritin L and H Subunits Are Differentially Regulated on a Post-transcriptional Level^*

From the Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112

Ferritin plays an important role in the storage and release of iron, an element utilized in cellular processes such as respiration, gene regulation, and DNA replication and repair. Ferritin in animals is composed of 24 ferritin L (FTL) and ferritin H (FTH) subunits in ratios that vary in different cell types. Because the subunits are not functionally interchangeable, both L and H units are critical for maintaining iron homeostasis and protecting against iron overload. FTL and FTH are regulated primarily at a post-transcriptional level in response to cellular iron concentrations. Individual regulation of FTL and FTH is of much interest, and although transcriptional differences between FTL and FTH have been shown, differences in their post-transcriptional regulation have not been evaluated. We report here that FTL and FTH are differentially regulated in 1% oxygen on a post-transcriptional level. We have designed a quantitative assay system sensitive enough to detect differences between FTL and FTH iron regulatory elements (IREs) that a standard electrophoretic mobility shift assay does not. The FTL IRE is the primary responder in the presence of an iron donor in hypoxic conditions, and this response is reflected in endogenous FTL protein levels. These results provide evidence that FTL and FTH subunits respond independently to cellular iron concentrations and underscore the importance of evaluating FTL and FTH IREs separately.

Received for publication, April 25, 2007 , and in revised form, December 21, 2007.

^* This work was supported in part by a grant from the Friedreich Ataxia Research Alliance. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Genetics, LSUHSC, 533 Bolivar St., New Orleans, LA 70112. Tel.: 504-568-6154; Fax: 504-568-8500; E-mail: egrabc{at}lsuhsc.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-F. Briat, K. Ravet, N. Arnaud, C. Duc, J. Boucherez, B. Touraine, F. Cellier, and F. Gaymard New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants Ann. Bot., May 29, 2009; (2009) mcp128v1. [Abstract] [Full Text] [PDF]

				R. Sengupta, S. Burbassi, S. Shimizu, S. Cappello, R. B. Vallee, J. B. Rubin, and O. Meucci Morphine Increases Brain Levels of Ferritin Heavy Chain Leading to Inhibition of CXCR4-Mediated Survival Signaling in Neurons J. Neurosci., February 25, 2009; 29(8): 2534 - 2544. [Abstract] [Full Text] [PDF]