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J. Biol. Chem., Vol. 283, Issue 9, 5306-5316, February 29, 2008
Both Gi and Go Heterotrimeric G Proteins Are Required to Exert the Full Effect of Norepinephrine on the β-Cell KATP Channel*![]() ![]() ![]() 1
From the
The effects of norepinephrine (NE), an inhibitor of insulin secretion, were examined on membrane potential and the ATP-sensitive K+ channel (KATP) in INS 832/13 cells. Membrane potential was monitored under the whole cell current clamp mode. NE hyperpolarized the cell membrane, an effect that was abolished by tolbutamide. The effect of NE on KATP channels was investigated in parallel using outside-out single channel recording. This revealed that NE enhanced the open activities of the KATP channels
Received for publication, September 13, 2007 , and in revised form, December 7, 2007. Note Added in Proof—Since the submission of this manuscript, a related article has been published (Katsuya, D., Kakei, M., and Yada, T. (2007) Diabetes 56, 2319–2327). * This work was supported by National Institutes of Health Grants DK54243 and DK56737 (to G. W. G. S.) and a Career Development Award from the Juvenile Diabetes Foundation International (to S. G. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 To whom correspondence should be addressed: Dept. of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. Tel.: 607-253-3875; Fax: 607-253-3659; E-mail: gws2{at}cornell.edu.
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