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J. Biol. Chem., Vol. 283, Issue 9, 5460-5465, February 29, 2008

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Identification of Integrin-4, Rb1, and Syncytin A as Murine Placental Target Genes of the Transcription Factor GCMa/Gcm1^*

Steffen Wolfgang Schubert, Nicolas Lamoureux, Karin Kilian, Ludger Klein-Hitpass, and Said Hashemolhosseini¹

From the Institut für Biochemie, Emil-Fischer-Zentrum, Universität Erlangen-Nürnberg, Fahrstr. 17, D-91054 Erlangen and the Institut für Zellbiologie, Universität Essen, Virchowstr. 173, D-45122 Essen, Germany

Members of the GCM (glial cells missing) transcription factor family have been shown to act as master regulators in different cells during mammalian and fly development being responsible for processes including gliogenesis, hematopoiesis, placental formation, and development of the parathyroidea. In the central nervous system of flies, several target genes for GCM have been reported, namely repo, pointed, and tramtrack. In mammals, two GCM genes are known (GCMa and GCMb), but the knowledge of their target genes is very limited. Here, we present for the first time a global approach aimed to identify GCMa target genes. We found 66 genes up-regulated and 11 genes down-regulated in GCMa-deficient chorionic tissue of mice at embryonic day 9.5. Moreover, we verified by quantitative reverse transcription-PCR all 11 down-regulated genes. The two most strongly down-regulated genes, integrin-4 and retinoblastoma (Rb1), were further analyzed by promoter studies. Additionally, we identified down-regulation of the murine syncytin A gene, which is fundamental for syncytiotrophoblast formation. Finally, we proved strong down-regulation of integrin-4 and Rb1 transcript levels by in situ hybridization in murine GCMa-deficient placentae at embryonic day 9.5. Our data demonstrate for the first time that genes encoding key regulators of placental tissue formation and architecture are regulated by GCMa.

Received for publication, December 12, 2007

^* This research was supported by grants of the Interdisciplinary Center for Clinical Research at the University Hospital of the University of Erlangen-Nuremberg (Grant IZKF A16) and Deutsche Forschungsgemeinschaft Center Grant SFB473 (to S. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains two supplemental tables.

¹ To whom correspondence should be addressed. Tel.: 49-9131-852-4634; Fax: 49-9131-852-2484; E-mail: sh{at}biochem.uni-erlangen.de.

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