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J. Biol. Chem., Vol. 284, Issue 2, 740-750, January 9, 2009

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Saccharomyces cerevisiae Linker Histone Hho1p Functionally Interacts with Core Histone H4 and Negatively Regulates the Establishment of Transcriptionally Silent Chromatin^*

Qun Yu, Holly Kuzmiak, Yanfei Zou, Lars Olsen, Pierre-Antoine Defossez¹, and Xin Bi²

From the Department of Biology, University of Rochester, Rochester, New York 14627 and Section Recherche, Institut Curie, CNRS UMR218, Paris 75248, France

Saccharomyces cerevisiae linker histone Hho1p is not essential for cell viability, and very little is known about its function in vivo. We show that deletion of HHO1 (hho1) suppresses the defect in transcriptional silencing caused by a mutation in the globular domain of histone H4. hho1 also suppresses the reduction in HML silencing by the deletion of SIR1 that is involved in the establishment of silent chromatin at HML. We further show that hho1 suppresses changes in silent chromatin structure caused by the histone H4 mutation and sir1. These results suggest that HHO1 plays a negative role in transcriptionally silent chromatin. We also provide evidence that Hho1p hinders the de novo establishment of silent chromatin but does not affect the stability of preexistent silent chromatin. Unlike canonical linker histones in higher eukaryotes that have a single conserved globular domain, Hho1p possesses two globular domains. We show that the carboxyl-terminal globular domain of Hho1p is dispensable for its function, suggesting that the mode of Hho1p action is similar to that of canonical linker histones.

Received for publication, August 14, 2008 , and in revised form, October 24, 2008.

^* This work was supported, in whole or in part, by National Institutes of Health Grant GM62484 (to X. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Supported by Centre National de la Recherche Scientifique Programme Action Thématique et Incitative sur Programme and by Fondation pour la Recherche Médicale.

² To whom correspondence should be addressed: Dept. of Biology, University of Rochester, Rochester, NY 14627. Tel.: 585-275-6922; Fax: 585-275-2070; E-mail: xinbi{at}mail.rochester.edu.

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