| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print August 31, 2000
National Institutes of Allergy & Infectious Diseases, Bethesda, MD 20892-0460
Corresponding Author: kj7e{at}nih.gov
Expression of the HTLV-I Tax oncoprotein rapidly engenders DNA-damage as reflected in a significant increase of micronuclei (MN) in cells. To understand better this phenomenon, we have investigated the DNA-content of MN induced by Tax. Using an approach which we termed FISHI, fluorescent in situ hybridization and incorporation, we attempted to characterize MN with centric or acentric DNA-fragments for the presence or absence of free 3?-OH ends. Free 3?-OH ends were defined as those ends accessible to in situ addition of digoxigenin (DIG)-dUTP using terminal deoxynucleotidyl transferase (TdT). MN were also assessed for centromeric sequences using standard fluorescent in situ hybridization (FISH). Combining these results, we determined that Tax oncoprotein increased the frequency of MN containing centric DNA with free 3?-OH and decreased the frequency of MN containing DNA fragments that had incorporation-inaccessible 3?-ends. Recently, it has been suggested that intracellular DNA breaks without detectable 3?-OH ends are stablilized by the protective addition of telomeric caps while breaks with freely detectable 3?-OH are uncapped and are labile to degradation, incomplete replication, and loss during cell division. Accordingly, based on increased detection of free 3?-OH-containing DNA-fragments, we concluded that HTLV-I Tax interferes with protective cellular mechanism(s) used normally for stabilizing DNA-breaks.
J. Biol. Chem, 10.1074/jbc.C000538200
Submitted on August 10, 2000
Accepted on August 30, 2000
Clastogenic effect of the Human T-cell leukemia virus type I Tax oncoprotein correlates with unstabilized DNA breaks
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Liu, L. Yang, L. Zhang, B. Liu, R. Merling, Z. Xia, and C.-Z. Giam Human T-Cell Leukemia Virus Type 1 Infection Leads to Arrest in the G1 Phase of the Cell Cycle J. Virol., September 1, 2008; 82(17): 8442 - 8455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Lindberg, G. C.-M. Falck, H. Jarventaus, and H. Norppa Characterization of chromosomes and chromosomal fragments in human lymphocyte micronuclei by telomeric and centromeric FISH Mutagenesis, September 1, 2008; 23(5): 371 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. T. Mihaylova, A. M. Green, M. Khurgel, O. J. Semmes, and G. M. Kupfer Human T-Cell Leukemia Virus I Tax Protein Sensitizes p53-Mutant Cells to DNA Damage Cancer Res., June 15, 2008; 68(12): 4843 - 4852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Gupta, X. Guo, S. S. Durkin, K. F. Fryrear, M. D. Ward, and O. J. Semmes Human T-cell Leukemia Virus Type 1 Tax Oncoprotein Prevents DNA Damage-induced Chromatin Egress of Hyperphosphorylated Chk2 J. Biol. Chem., October 5, 2007; 282(40): 29431 - 29440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Durkin, M. D. Ward, K. A. Fryrear, and O. J. Semmes Site-specific Phosphorylation Differentiates Active from Inactive Forms of the Human T-cell Leukemia Virus Type 1 Tax Oncoprotein J. Biol. Chem., October 20, 2006; 281(42): 31705 - 31712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gatza and S. J. Marriott Genotoxic Stress and Cellular Stress Alter the Subcellular Distribution of Human T-Cell Leukemia Virus Type 1 Tax through a CRM1-Dependent Mechanism. J. Virol., July 1, 2006; 80(13): 6657 - 6668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Peloponese Jr. and K.-T. Jeang Role for Akt/Protein Kinase B and Activator Protein-1 in Cellular Proliferation Induced by the Human T-cell Leukemia Virus Type 1 Tax Oncoprotein J. Biol. Chem., March 31, 2006; 281(13): 8927 - 8938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bolognesi, F. Martini, M. Tognon, R. Filiberti, M. Neri, E. Perrone, E. Landini, P. A. Canessa, G. P. Ivaldi, P. Betta, et al. A Molecular Epidemiology Case Control Study on Pleural Malignant Mesothelioma Cancer Epidemiol. Biomarkers Prev., July 1, 2005; 14(7): 1741 - 1746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Peloponese Jr., H. Iha, V. R. K. Yedavalli, A. Miyazato, Y. Li, K. Haller, M. Benkirane, and K.-T. Jeang Ubiquitination of Human T-Cell Leukemia Virus Type 1 Tax Modulates Its Activity J. Virol., November 1, 2004; 78(21): 11686 - 11695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yoshizuka, R. Moriuchi, T. Mori, K. Yamada, S. Hasegawa, T. Maeda, T. Shimada, Y. Yamada, S. Kamihira, M. Tomonaga, et al. An Alternative Transcript Derived from the Trio Locus Encodes a Guanosine Nucleotide Exchange Factor with Mouse Cell-transforming Potential J. Biol. Chem., October 15, 2004; 279(42): 43998 - 44004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-T. Jeang, C.-z. Giam, F. Majone, and M. Aboud Life, Death, and Tax: Role of HTLV-I Oncoprotein in Genetic Instability and Cellular Transformation J. Biol. Chem., July 30, 2004; 279(31): 31991 - 31994. [Full Text] [PDF]
|
|
|
|
 |
|
 X. Jiang, Y. Zhao, W.-Y. Chan, S. Vercauteren, E. Pang, S. Kennedy, F. Nicolini, A. Eaves, and C. Eaves Deregulated expression in Ph+ human leukemias of AHI-1, a gene activated by insertional mutagenesis in mouse models of leukemia Blood, May 15, 2004; 103(10): 3897 - 3904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-C. Twizere, V. Kruys, L. Lefebvre, A. Vanderplasschen, D. Collete, C. Debacq, W. S. Lai, J.-C. Jauniaux, L. R. Bernstein, O. J. Semmes, et al. Interaction of Retroviral Tax Oncoproteins With Tristetraprolin and Regulation of Tumor Necrosis Factor-{alpha} Expression J Natl Cancer Inst, December 17, 2003; 95(24): 1846 - 1859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Norppa and G. C.-M. Falck What do human micronuclei contain? Mutagenesis, May 1, 2003; 18(3): 221 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Lee, B.-C. Kim, J. N. Brady, K.-T. Jeang, and S.-J. Kim Human T-cell Lymphotropic Virus Type 1 Tax Inhibits Transforming Growth Factor-beta Signaling by Blocking the Association of Smad Proteins with Smad-binding Element J. Biol. Chem., September 6, 2002; 277(37): 33766 - 33775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Liang, T. Geisbert, Y. Yao, S. H. Hinrichs, and C.-Z. Giam Human T-Lymphotropic Virus Type 1 Oncoprotein Tax Promotes S-Phase Entry but Blocks Mitosis J. Virol., March 19, 2002; 76(8): 4022 - 4033. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
