JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on August 17, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/34/31475    most recent
C100238200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adachi, J.
Right arrow Articles by Matsuda, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adachi, J.
Right arrow Articles by Matsuda, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print June 25, 2001
J. Biol. Chem, 10.1074/jbc.C100238200
Submitted on May 10, 2001
Revised on June 1, 2001
Accepted on June 25, 2001

Indirubin and indigo are potent Aryl hydrocarbon receptor ligands present in human urine

Jun Adachi, Yoshitomo Mori, Saburo Matsui, Hidetaka Takigami, Junko Fujino, Hiroko Kitagawa, Charles A. Miller III, Takaaki Kato, Kenichi Saeki, and Tomonari Matsuda

Resaerch Center for Environmental Quality Control, Kyoto University, Otsu, Shiga 520-0811

Corresponding Author: matsuda{at}biwa.eqc.kyoto-u.ac.jp

AhR is a ligand-activated transcription factor that regulates genes involved in xenobiotic metabolism, cellular proliferation, and differentiation. Numerous xenobiotic and biological compounds are known to interact with AhR, but it remains an orphan receptor since its physiological ligand is unknown. We identified AhR ligands in human urine using a yeast AhR signaling assay and then characterized their properties. Two ligands, indirubin and indigo, were both present at average concentrations of approximately 0.2 nM in the urine of normal donors. Indirubin was also detected in fetal bovine serum and contributed half of the total AhR ligand activity. The activities of indirubin and indigo were comparable to or more potent than that of the archetypal ligand, TCDD, in yeast AhR activation assays. We suggest that the endogenous levels and potencies of indirubin and indigo are such that they activate AhR-mediated signaling mechanisms in vivo.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
B. J. McMillan and C. A. Bradfield
The Aryl Hydrocarbon Receptor sans Xenobiotics: Endogenous Function in Genetic Model Systems
Mol. Pharmacol., September 1, 2007; 72(3): 487 - 498.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
D. Nestler, M. Risch, B. Fischer, and P. Pocar
Regulation of aryl hydrocarbon receptor activity in porcine cumulus-oocyte complexes in physiological and toxicological conditions: the role of follicular fluid
Reproduction, May 1, 2007; 133(5): 887 - 897.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. Mukai and S. A. Tischkau
Effects of Tryptophan Photoproducts in the Circadian Timing System: Searching for a Physiological Role for Aryl Hydrocarbon Receptor
Toxicol. Sci., January 1, 2007; 95(1): 172 - 181.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Sethi, K. S. Ahn, S. K. Sandur, X. Lin, M. M. Chaturvedi, and B. B. Aggarwal
Indirubin Enhances Tumor Necrosis Factor-induced Apoptosis through Modulation of Nuclear Factor-{kappa}B Signaling Pathway
J. Biol. Chem., August 18, 2006; 281(33): 23425 - 23435.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. L. Petersen, S. Krishnan, and E. D. Hudgens
The Aryl Hydrocarbon Receptor Pathway and Sexual Differentiation of Neuroendocrine Functions
Endocrinology, June 1, 2006; 147(6): s33 - s42.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. Diani-Moore, E. Labitzke, R. Brown, A. Garvin, L. Wong, and A. B. Rifkind
Sunlight Generates Multiple Tryptophan Photoproducts Eliciting High Efficacy CYP1A Induction in Chick Hepatocytes and In Vivo
Toxicol. Sci., March 1, 2006; 90(1): 96 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
U. A. Bussmann, L. E. Bussmann, and J. L. Baranao
An Aryl Hydrocarbon Receptor Agonist Amplifies the Mitogenic Actions of Estradiol in Granulosa Cells: Evidence of Involvement of the Cognate Receptors
Biol Reprod, February 1, 2006; 74(2): 417 - 426.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Mulero-Navarro, E. Pozo-Guisado, P. A. Perez-Mancera, A. Alvarez-Barrientos, I. Catalina-Fernandez, E. Hernandez-Nieto, J. Saenz-Santamaria, N. Martinez, J. M. Rojas, I. Sanchez-Garcia, et al.
Immortalized Mouse Mammary Fibroblasts Lacking Dioxin Receptor Have Impaired Tumorigenicity in a Subcutaneous Mouse Xenograft Model
J. Biol. Chem., August 5, 2005; 280(31): 28731 - 28741.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
P Pocar, B Fischer, T Klonisch, and S Hombach-Klonisch
Molecular interactions of the aryl hydrocarbon receptor and its biological and toxicological relevance for reproduction
Reproduction, April 1, 2005; 129(4): 379 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. Adachi, Y. Mori, S. Matsui, and T. Matsuda
Comparison of Gene Expression Patterns between 2,3,7,8-Tetrachlorodibenzo-p-dioxin and a Natural Arylhydrocarbon Receptor Ligand, Indirubin
Toxicol. Sci., July 1, 2004; 80(1): 161 - 169.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ikuta, Y. Kobayashi, and K. Kawajiri
Cell Density Regulates Intracellular Localization of Aryl Hydrocarbon Receptor
J. Biol. Chem., April 30, 2004; 279(18): 19209 - 19216.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
L. Andrieux, S. Langouet, A. Fautrel, F. Ezan, J. A. Krauser, J. F. Savouret, F. P. Guengerich, G. Baffet, and A. Guillouzo
Aryl Hydrocarbon Receptor Activation and Cytochrome P450 1A Induction by the Mitogen-Activated Protein Kinase Inhibitor U0126 in Hepatocytes
Mol. Pharmacol., April 1, 2004; 65(4): 934 - 943.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
P. Pocar, R. Augustin, and B. Fischer
Constitutive Expression of CYP1A1 in Bovine Cumulus Oocyte-Complexes in Vitro: Mechanisms and Biological Implications
Endocrinology, April 1, 2004; 145(4): 1594 - 1601.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Yang, F. Boucher, A. Tremblay, and J. L. Michaud
Regulatory Interaction between Arylhydrocarbon Receptor and SIM1, Two Basic Helix-Loop-Helix PAS Proteins Involved in the Control of Food Intake
J. Biol. Chem., March 5, 2004; 279(10): 9306 - 9312.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
K. P. Lai, M. H. Wong, and C. K. C. Wong
Modulation of AhR-Mediated CYP1A1 mRNA and EROD Activities by 17{beta}-Estradiol and Dexamethasone in TCDD-Induced H411E Cells
Toxicol. Sci., March 1, 2004; 78(1): 41 - 49.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. Levine-Fridman, L. Chen, and C. J. Elferink
Cytochrome P4501A1 Promotes G1 Phase Cell Cycle Progression by Controlling Aryl Hydrocarbon Receptor Activity
Mol. Pharmacol., February 1, 2004; 65(2): 461 - 469.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
R. Sakai, T. Kajiume, H. Inoue, R. Kanno, M. Miyazaki, Y. Ninomiya, and M. Kanno
TCDD Treatment Eliminates the Long-Term Reconstitution Activity of Hematopoietic Stem Cells
Toxicol. Sci., March 1, 2003; 72(1): 84 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
E. C. Henry and T. A. Gasiewicz
Agonist but Not Antagonist Ligands Induce Conformational Change in the Mouse Aryl Hydrocarbon Receptor as Detected by Partial Proteolysis
Mol. Pharmacol., February 1, 2003; 63(2): 392 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. D. Maines
Bile Pigments: Newcomers to the Cell Signaling Arena
Toxicol. Sci., January 1, 2003; 71(1): 9 - 10.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Song, M. Clagett-Dame, R. E. Peterson, M. E. Hahn, W. M. Westler, R. R. Sicinski, and H. F. DeLuca
A ligand for the aryl hydrocarbon receptor isolated from lung
PNAS, November 12, 2002; 99(23): 14694 - 14699.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.