JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 1, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/10/7641    most recent
C100737200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, H.-P.
Right arrow Articles by Warnock, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, H.-P.
Right arrow Articles by Warnock, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 23, 2002
J. Biol. Chem, 10.1074/jbc.C100737200
Submitted on December 17, 2001
Revised on January 23, 2002
Accepted on January 17, 2002

Anionic phospholipids regulate native and expressed ENaC

He-Ping Ma, Sunil Saxena, and David G. Warnock

Medicine/Nephrology, University of Alabama at Birmingham, Birmingham, Alabama 35294-0017

Corresponding Author: hepingma{at}uab.edu

Using patch-clamp techniques, we found that ENaC activity in the apical membrane of A6 distal nephron cells showed a sudden rundown beginning at 4 min after forming the inside-out configuration. This sudden rundown was prevented by addition of anionic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 3,4,5-trisphosphate (PIP3), and phosphatidylserine (PS) to the “cytoplasmic” bath. Conversely, chelation of endogenous PIP2 with anti-PIP2 antibody, hydrolysis of PIP2 with either exogenous phospholipase C (PLC) or activation of endogenous PLC by extracellular ATP, or application of positively charged molecule, poly-L-lysine, accelerated channel rundown. However, neutral phosphatidylcholine (PC) had no effect on ENaC activity. By two-electrode voltage-clamp recordings, we demonstrated that PIP2 and PIP3 significantly increased amiloride-sensitive current in Xenopus oocytes injected with cRNAs of rat alpha -, beta -, and gamma -ENaC. However, PIP2 and PIP3 did not affect surface expression of ENaC, indicating that PIP2 and PIP3 regulate ENaC at the level of the inner plasma membrane through a mechanism that is independent of ENaC trafficking. These data suggest that anionic phospholipids may mediate the regulation of ENaC by PLC- or PI 3-kinase-coupled receptors.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
A. P. Albert, S. N. Saleh, and W. A. Large
Inhibition of native TRPC6 channel activity by phosphatidylinositol 4,5-bisphosphate in mesenteric artery myocytes
J. Physiol., July 1, 2008; 586(13): 3087 - 3095.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. Pochynyuk, V. Bugaj, A. Vandewalle, and J. D. Stockand
Purinergic control of apical plasma membrane PI(4,5)P2 levels sets ENaC activity in principal cells
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F38 - F46.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. Vallon
P2 receptors in the regulation of renal transport mechanisms
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Li, C. S. Wingo, and S.-L. Xia
Downregulation of SGK1 by nucleotides in renal tubular epithelial cells
Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1751 - F1757.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H.-F. Bao, Z.-R. Zhang, Y.-Y. Liang, J. J. Ma, D. C. Eaton, and H.-P. Ma
Ceramide mediates inhibition of the renal epithelial sodium channel by tumor necrosis factor-{alpha} through protein kinase C
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1178 - F1186.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
O. Pochynyuk, Q. Tong, J. Medina, A. Vandewalle, A. Staruschenko, V. Bugaj, and J. D. Stockand
Molecular Determinants of PI(4,5)P2 and PI(3,4,5)P3 Regulation of the Epithelial Na+ Channel
J. Gen. Physiol., September 24, 2007; 130(4): 399 - 413.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
X. Xu, A. Muller-Taubenberger, K. E. Adley, N. Pawolleck, V. W. Y. Lee, C. Wiedemann, T. S. Sihra, M. Maniak, T. Jin, and R. S. B. Williams
Attenuation of Phospholipid Signaling Provides a Novel Mechanism for the Action of Valproic Acid
Eukaryot. Cell, June 1, 2007; 6(6): 899 - 906.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
O. Pochynyuk, Q. Tong, A. Staruschenko, and J. D. Stockand
Binding and direct activation of the epithelial Na+ channel (ENaC) by phosphatidylinositides
J. Physiol., April 15, 2007; 580(2): 365 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. Pochynyuk, Q. Tong, A. Staruschenko, H.-P. Ma, and J. D. Stockand
Regulation of the epithelial Na+ channel (ENaC) by phosphatidylinositides
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F949 - F957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. N. Helms, X.-J. Chen, S. Ramosevac, D. C. Eaton, and L. Jain
Dopamine regulation of amiloride-sensitive sodium channels in lung cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L710 - L722.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Li, Y. Wei, E. Babilonia, Z. Wang, and W.-H. Wang
Inhibition of phosphatidylinositol 3-kinase stimulates activity of the small-conductance K channel in the CCD
Am J Physiol Renal Physiol, April 1, 2006; 290(4): F806 - F812.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. N. Helms, L. Liu, Y.-Y. Liang, O. Al-Khalili, A. Vandewalle, S. Saxena, D. C. Eaton, and H.-P. Ma
Phosphatidylinositol 3,4,5-Trisphosphate Mediates Aldosterone Stimulation of Epithelial Sodium Channel (ENaC) and Interacts with {gamma}-ENaC
J. Biol. Chem., December 9, 2005; 280(49): 40885 - 40891.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Pochynyuk, A. Staruschenko, Q. Tong, J. Medina, and J. D. Stockand
Identification of a Functional Phosphatidylinositol 3,4,5-Trisphosphate Binding Site in the Epithelial Na+ Channel
J. Biol. Chem., November 11, 2005; 280(45): 37565 - 37571.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H.-P. Ma and D. C. Eaton
Acute Regulation of Epithelial Sodium Channel by Anionic Phospholipids
J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3182 - 3187.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Bachhuber, J. Konig, T. Voelcker, B. Murle, R. Schreiber, and K. Kunzelmann
Cl- Interference with the Epithelial Na+ Channel ENaC
J. Biol. Chem., September 9, 2005; 280(36): 31587 - 31594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. C. Wagner, B. L. Blazer-Yost, J. Boyd-White, A. Srirangam, J. Pennington, and S. Bennett
Expression of the unconventional myosin Myo1c alters sodium transport in M1 collecting duct cells
Am J Physiol Cell Physiol, July 1, 2005; 289(1): C120 - C129.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Tong and J. D. Stockand
Receptor tyrosine kinases mediate epithelial Na+ channel inhibition by epidermal growth factor
Am J Physiol Renal Physiol, January 1, 2005; 288(1): F150 - F161.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Staruschenko, A. Nichols, J. L. Medina, P. Camacho, N. N. Zheleznova, and J. D. Stockand
Rho Small GTPases Activate the Epithelial Na+ Channel
J. Biol. Chem., November 26, 2004; 279(48): 49989 - 49994.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Mies, V. Shlyonsky, A. Goolaerts, and S. Sariban-Sohraby
Modulation of epithelial Na+ channel activity by long-chain n-3 fatty acids
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F850 - F855.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Staruschenko, P. Patel, Q. Tong, J. L. Medina, and J. D. Stockand
Ras Activates the Epithelial Na+ Channel through Phosphoinositide 3-OH Kinase Signaling
J. Biol. Chem., September 3, 2004; 279(36): 37771 - 37778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. Markadieu, D. Blero, A. Boom, C. Erneux, and R. Beauwens
Phosphatidylinositol 3,4,5-trisphosphate: an early mediator of insulin-stimulated sodium transport in A6 cells
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F319 - F328.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Berdiev, B. Jovov, W. C. Tucker, A. P. Naren, C. M. Fuller, E. R. Chapman, and D. J. Benos
ENaC subunit-subunit interactions and inhibition by syntaxin 1A
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1100 - F1106.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Tong, R. E. Booth, R. T. Worrell, and J. D. Stockand
Regulation of Na+ transport by aldosterone: signaling convergence and cross talk between the PI3-K and MAPK1/2 cascades
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1232 - F1238.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Tong, N. Gamper, J. L. Medina, M. S. Shapiro, and J. D. Stockand
Direct Activation of the Epithelial Na+ Channel by Phosphatidylinositol 3,4,5-Trisphosphate and Phosphatidylinositol 3,4-Bisphosphate Produced by Phosphoinositide 3-OH Kinase
J. Biol. Chem., May 21, 2004; 279(21): 22654 - 22663.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
P. Huang, E. Gilmore, P. Kultgen, P. Barnes, S. Milgram, and M. J. Stutts
Local Regulation of Cystic Fibrosis Transmembrane Regulator and Epithelial Sodium Channel in Airway Epithelium
Proceedings of the ATS, January 1, 2004; 1(1): 33 - 37.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. E. Booth, Q. Tong, J. Medina, P. M. Snyder, P. Patel, and J. D. Stockand
A Region Directly Following the Second Transmembrane Domain in {gamma}ENaC Is Required for Normal Channel Gating
J. Biol. Chem., October 17, 2003; 278(42): 41367 - 41379.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. L. Blazer-Yost, M. A. Esterman, and C. J. Vlahos
Insulin-stimulated trafficking of ENaC in renal cells requires PI 3-kinase activity
Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1645 - C1653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. G. Shlyonsky, F. Mies, and S. Sariban-Sohraby
Epithelial sodium channel activity in detergent-resistant membrane microdomains
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F182 - F188.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Okamoto, S. Vaena de Avalos, and Y. A. Hannun
Structural Requirements for Selective Binding of ISC1 to Anionic Phospholipids
J. Biol. Chem., November 22, 2002; 277(48): 46470 - 46477.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.