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A more recent version of this article appeared on April 19, 2002
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C200101200v1
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Papers In Press, published online ahead of print March 6, 2002
J. Biol. Chem, 10.1074/jbc.C200101200
Submitted on February 15, 2002
Revised on March 4, 2002
Accepted on March 6, 2002

Transcript abundance in yeast varies over six orders of magnitude

Michael J. Holland

Biological Chemistry, University of California, Davis, CA 95616

Corresponding Author: mjholland{at}ucdavis.edu

In the current era of functional genomics, it is remarkable that the intracellular range of transcript abundance is largely unknown. For the yeast Saccharomyces cerevisiae, hybridization-based complexity analysis and SAGE analysis showed that the majority of yeast mRNAs are present at one or fewer copies per cell, however, neither method provides an accurate estimate of the full range of low abundance transcripts. Here we examine the range of intracellular transcript abundance in yeast using kinetically-monitored, reverse transcriptase initiated, PCR (kRT-PCR). Steady-state transcript levels encoded by all 65 genes on the left arm of chromosome III and 185 transcription factor genes are quantitated. Abundant transcripts encoded by glycolytic genes, previously quantitated by kRT-PCR, are present at a few hundred copies per cell whereas genes encoding physiologically important transcription factors are expressed at levels as low as a thousandth of a transcript per cell. Of the genes assessed, only the silent mating type loci, HML and HMR, are transcriptionally silent. The results show that transcript abundance in yeast varies over six orders of magnitude. Finally, kRT-PCR, cDNA microarray, and high-density oligonucleotide array assays are compared for their ability to detect and quantitate the complete yeast transcriptome.


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