JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 14, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/25/22111    most recent
C200151200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fong, A.
Right arrow Articles by Sun, S.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fong, A.
Right arrow Articles by Sun, S.-C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 6, 2002
J. Biol. Chem, 10.1074/jbc.C200151200
Submitted on March 14, 2002
Revised on May 2, 2002
Accepted on May 3, 2002

Genetic evidence for the essential role of beta-transducin repeat-containing protein in the inducible processing of NF-kB2/p100

Abraham Fong and Shao-Cong Sun

Microbiology and Immunology, Penn State University College of Medicine, Hershey, PA 17033

Corresponding Author: sxs70{at}psu.edu

Processing of the nfkb2 gene product p100 to generate p52 is an important step in NF-kB regulation. This step is regulated by a nonclassical NF-kB signaling pathway involving the NF-kB inducing kinase (NIK). NIK induces p100 processing by triggering phosphorylation of specific C-terminal serines of p100. However, the downstream molecular events leading to p100 processing remain unclear. Here we show that NIK induces the physical recruitment of beta-transducin repeat-containing protein (b-TrCP), a component of the SCF ubiquitin ligase complex, to p100. This event requires the phosphorylation sites as well as the death domain of p100. Using the RNA interference (RNAi) technique, we demonstrate that b-TrCP is essential for NIK-induced p100 ubiquitination and processing. Interestingly, the constitutive processing of p100 mutants is independent of b-TrCP. These results suggest that b-TrCP is an essential component of NIK-induced p100 processing.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. J. Fusco, O. V. Savinova, R. Talwar, J. D. Kearns, A. Hoffmann, and G. Ghosh
Stabilization of RelB Requires Multidomain Interactions with p100/p52
J. Biol. Chem., May 2, 2008; 283(18): 12324 - 12332.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Tucker, K. O'Donnell, M. Fuchsberger, A. A. Hilton, D. Metcalf, K. Greig, N. A. Sims, J. M. Quinn, W. S. Alexander, D. J. Hilton, et al.
A Novel Mutation in the Nfkb2 Gene Generates an NF-{kappa}B2 "Super Repressor"
J. Immunol., December 1, 2007; 179(11): 7514 - 7522.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W.-G. Deng, S.-T. Tang, H.-P. Tseng, and K. K. Wu
Melatonin suppresses macrophage cyclooxygenase-2 and inducible nitric oxide synthase expression by inhibiting p52 acetylation and binding
Blood, July 15, 2006; 108(2): 518 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Tempe, M. Casas, S. Karaz, M.-F. Blanchet-Tournier, and J.-P. Concordet
Multisite Protein Kinase A and Glycogen Synthase Kinase 3{beta} Phosphorylation Leads to Gli3 Ubiquitination by SCF{beta}TrCP.
Mol. Cell. Biol., June 1, 2006; 26(11): 4316 - 4326.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Pan, C. B. Bai, A. L. Joyner, and B. Wang
Sonic hedgehog Signaling Regulates Gli2 Transcriptional Activity by Suppressing Its Processing and Degradation.
Mol. Cell. Biol., May 1, 2006; 26(9): 3365 - 3377.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Wang and Y. Li
Evidence for the direct involvement of {beta}TrCP in Gli3 protein processing
PNAS, January 3, 2006; 103(1): 33 - 38.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Qing, Z. Qu, and G. Xiao
Stabilization of Basally Translated NF-{kappa}B-inducing Kinase (NIK) Protein Functions as a Molecular Switch of Processing of NF-{kappa}B2 p100
J. Biol. Chem., December 9, 2005; 280(49): 40578 - 40582.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
K. S. Richardson and W. Zundel
The Emerging Role of the COP9 Signalosome in Cancer
Mol. Cancer Res., December 1, 2005; 3(12): 645 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Wan, J. Huang, N. C. Jhala, E. M. Tytler, L. Yang, S. M. Vickers, Y. Tang, C. Lu, N. Wang, and X. Cao
SCF{beta}-TrCP1 Controls Smad4 Protein Stability in Pancreatic Cancer Cells
Am. J. Pathol., May 1, 2005; 166(5): 1379 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Qing and G. Xiao
Essential Role of I{kappa}B Kinase {alpha} in the Constitutive Processing of NF-{kappa}B2 p100
J. Biol. Chem., March 18, 2005; 280(11): 9765 - 9768.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. Hu and N. H. Colburn
Histone Deacetylase Inhibition Down-Regulates Cyclin D1 Transcription by Inhibiting Nuclear Factor-{kappa}B/p65 DNA Binding
Mol. Cancer Res., February 1, 2005; 3(2): 100 - 109.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Qing, Z. Qu, and G. Xiao
Regulation of NF-{kappa}B2 p100 Processing by Its cis-Acting Domain
J. Biol. Chem., January 7, 2005; 280(1): 18 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Shcherbik, Y. Kee, N. Lyon, J. M. Huibregtse, and D. S. Haines
A Single PXY Motif Located within the Carboxyl Terminus of Spt23p and Mga2p Mediates a Physical and Functional Interaction with Ubiquitin Ligase Rsp5p
J. Biol. Chem., December 17, 2004; 279(51): 53892 - 53898.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Qu, G. Qing, A. Rabson, and G. Xiao
Tax Deregulation of NF-{kappa}B2 p100 Processing Involves Both {beta}-TrCP-dependent and -independent Mechanisms
J. Biol. Chem., October 22, 2004; 279(43): 44563 - 44572.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Kudo, D. Guardavaccaro, P. G. Santamaria, R. Koyama-Nasu, E. Latres, R. Bronson, L. Yamasaki, and M. Pagano
Role of F-Box Protein {beta}Trcp1 in Mammary Gland Development and Tumorigenesis
Mol. Cell. Biol., September 15, 2004; 24(18): 8184 - 8194.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. S. Hayden and S. Ghosh
Signaling to NF-{kappa}B
Genes & Dev., September 15, 2004; 18(18): 2195 - 2224.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W.-H. Hu, X.-M. Mo, W. M. Walters, R. Brambilla, and J. R. Bethea
TNAP, a Novel Repressor of NF-{kappa}B-inducing Kinase, Suppresses NF-{kappa}B Activation
J. Biol. Chem., August 20, 2004; 279(34): 35975 - 35983.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
A. Ougolkov, B. Zhang, K. Yamashita, V. Bilim, M. Mai, S. Y. Fuchs, and T. Minamoto
Associations Among {beta}-TrCP, an E3 Ubiquitin Ligase Receptor, {beta}-Catenin, and NF-{kappa}B in Colorectal Cancer
J Natl Cancer Inst, August 4, 2004; 96(15): 1161 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Xiao, A. Fong, and S.-C. Sun
Induction of p100 Processing by NF-{kappa}B-inducing Kinase Involves Docking I{kappa}B Kinase {alpha} (IKK{alpha}) to p100 and IKK{alpha}-mediated Phosphorylation
J. Biol. Chem., July 16, 2004; 279(29): 30099 - 30105.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Besnard-Guerin, N. Belaidouni, I. Lassot, E. Segeral, A. Jobart, C. Marchal, and R. Benarous
HIV-1 Vpu Sequesters {beta}-Transducin Repeat-containing Protein ({beta}TrCP) in the Cytoplasm and Provokes the Accumulation of {beta}-Catenin and Other SCF{beta}TrCP Substrates
J. Biol. Chem., January 2, 2004; 279(1): 788 - 795.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Cohen, H. Achbert-Weiner, and A. Ciechanover
Dual Effects of I{kappa}B Kinase {beta}-Mediated Phosphorylation on p105 Fate: SCF{beta}-TrCP-Dependent Degradation and SCF{beta}-TrCP-Independent Processing
Mol. Cell. Biol., January 1, 2004; 24(1): 475 - 486.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. Jin, T. Shirogane, L. Xu, G. Nalepa, J. Qin, S. J. Elledge, and J. W. Harper
SCF{beta}-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
Genes & Dev., December 15, 2003; 17(24): 3062 - 3074.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mantovani and L. Banks
Regulation of the Discs Large Tumor Suppressor by a Phosphorylation-dependent Interaction with the {beta}-TrCP Ubiquitin Ligase Receptor
J. Biol. Chem., October 24, 2003; 278(43): 42477 - 42486.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
I. Dozmorov, M. R. Saban, N. P. Gerard, B. Lu, N.-B. Nguyen, M. Centola, and R. Saban
Neurokinin 1 receptors and neprilysin modulation of mouse bladder gene regulation
Physiol Genomics, February 6, 2003; 12(3): 239 - 250.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Fong, M. Zhang, J. Neely, and S.-C. Sun
S9, a 19 S Proteasome Subunit Interacting with Ubiquitinated NF-kappa B2/p100
J. Biol. Chem., October 18, 2002; 277(43): 40697 - 40702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. S. Spiegelman, W. Tang, A. M. Chan, M. Igarashi, S. A. Aaronson, D. A. Sassoon, M. Katoh, T. J. Slaga, and S. Y. Fuchs
Induction of Homologue of Slimb Ubiquitin Ligase Receptor by Mitogen Signaling
J. Biol. Chem., September 20, 2002; 277(39): 36624 - 36630.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.