JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 10, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
280/23/22492    most recent
C500122200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, Y.
Right arrow Articles by Wilson, K. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, Y.
Right arrow Articles by Wilson, K. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print April 20, 2005
J. Biol. Chem, 10.1074/jbc.C500122200
Submitted on March 21, 2005
Revised on April 13, 2005
Accepted on April 20, 2005

Helicobacter pylori-induced macrophage apoptosis requires activation of ornithine decarboxylase by c-Myc

Yulan Cheng, Rupesh Chaturvedi, Mohammad Asim, Francoise I. Bussiere, Hangxiu Xu, Robert A. Casero . Jr, and Keith T. Wilson

Medicine Dept., Division of Gastroenterology, University of Maryland School of Medicine, Baltimore, MD 21201

Corresponding Author: kwilson{at}umaryland.edu

Helicobacter pylori infection causes chronic inflammation of the gastric mucosa that results from an ineffective immune response. We have demonstrated that one underlying mechanism is induction of macrophage apoptosis mediated by polyamines. The transcription factor c-Myc has been linked to induction of ornithine decarboxylase (ODC), the rate limiting enzyme in polyamine synthesis. We determined whether H. pylori stimulates transcriptional activation of ODC in macrophages, if this occurs via c-Myc, and if these events regulate activation of apoptosis. H. pylori induced a significant increase in ODC promoter activity that peaked at 6 h after stimulation and was closely paralleled by similar increases in ODC mRNA, protein, and enzyme activity. By 2 h after stimulation, c-Myc mRNA and protein expression was induced, protein translocated to the nucleus, and there was specific binding of a consensus probe for c-Myc to nuclear extracts. Both an antennapedia-linked inhibitor of c-Myc binding (Int-H1-S6A, F8A) and transfection of a c-Myc dominant-negative construct significantly attenuated H. pylori-induced ODC promoter activity, mRNA, enzyme activity, and apoptosis in parallel. Transfection of ODC small interfering RNA inhibited ODC activity and apoptosis to the same degree as inhibition of c-Myc binding. These studies indicate that c-Myc is an important mediator of macrophage activation and may contribute to the mucosal inflammatory response to pathogens such as H. pylori by its effect on ODC.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Singh, R. Chaturvedi, M. Asim, D. P. Barry, N. D. Lewis, M. P. Vitek, and K. T. Wilson
The Apolipoprotein E-mimetic Peptide COG112 Inhibits the Inflammatory Response to Citrobacter rodentium in Colonic Epithelial Cells by Preventing NF-{kappa}B Activation
J. Biol. Chem., June 13, 2008; 283(24): 16752 - 16761.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
T. Kurita-Ochiai, S. Seto, N. Suzuki, M. Yamamoto, K. Otsuka, K. Abe, and K. Ochiai
Butyric Acid Induces Apoptosis in Inflamed Fibroblasts
J. Dent. Res., January 1, 2008; 87(1): 51 - 55.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
I. Popa, I. Zubkova, M. Medvedovic, T. Romantseva, H. Mostowski, R. Boyd, and M. Zaitseva
Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc and Tcf3 transcription factors in the thymic stroma
Int. Immunol., November 1, 2007; 19(11): 1249 - 1260.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Chaturvedi, M. Asim, N. D. Lewis, H. M. S. Algood, T. L. Cover, P. Y. Kim, and K. T. Wilson
L-Arginine Availability Regulates Inducible Nitric Oxide Synthase-Dependent Host Defense against Helicobacter pylori
Infect. Immun., September 1, 2007; 75(9): 4305 - 4315.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
H. M. S. Algood and T. L. Cover
Helicobacter pylori Persistence: an Overview of Interactions between H. pylori and Host Immune Defenses
Clin. Microbiol. Rev., October 1, 2006; 19(4): 597 - 613.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. I. Bussiere, R. Chaturvedi, M. Asim, K. L. Hoek, Y. Cheng, J. Gainor, A. Scholz, W. N. Khan, and K. T. Wilson
Low Multiplicity of Infection of Helicobacter pylori Suppresses Apoptosis of B Lymphocytes.
Cancer Res., July 1, 2006; 66(13): 6834 - 6842.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.