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Papers In Press, published online ahead of print June 21, 2000
LMB, NIDDK, NIH, Bethesda, MD 20892
Corresponding Author: ceceliat{at}intra.niddk.nih.gov
GATA-1 and other vertebrate GATA factors contain a DNA binding domain composed of two adjacent homologous zinc fingers. Whereas only the C-terminal finger of GATA-1 is capable of independent binding to the GATA recognition sequence, double GATA sites which require both fingers for high affinity interaction are found in several genes. We propose a mechanism whereby adjacent zinc fingers interact to influence the binding and transactivation properties of GATA-1 at a subset of DNA binding sites. Using two such double GATA sites we demonstrate that the N-terminal finger and adjacent linker region can alter the binding specificity of the C-terminal finger sufficiently to prevent it from recognizing some consensus GATA sequences. Therefore, the two zinc fingers form a composite binding domain having a different DNA-binding specificity from that shown by the constituent single C-terminal finger. Furthermore, we compare two of these double sites and show that high affinity binding of GATA-1 to a reporter gene does not necessarily induce transactivation, namely, the sequence of the DNA binding site can alter the ability of GATA-1 to stimulate transcription.
J. Biol. Chem, 10.1074/jbc.M000020200
Submitted on January 3, 2000
Revised on June 14, 2000
Accepted on June 20, 2000
GATA Zinc Finger Interactions Modulate DNA Binding and Transactivation
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