JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 14, 2000
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
275/29/22284    most recent
M000118200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paine, C. T
Right arrow Articles by Snead, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paine, C. T
Right arrow Articles by Snead, M. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 9, 2000
J. Biol. Chem, 10.1074/jbc.M000118200
Submitted on January 7, 2000
Revised on May 2, 2000
Accepted on May 8, 2000

A tuftelin-interacting protein (TIP39) localized to the apical secretory pole of mouse ameloblasts

Caroline T Paine, Michael L Paine, Wen Luo, Curtis T Okamoto, Staele Petter Lyngstadaas, and Malcolm L. Snead

Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033

Corresponding Author: mlsnead{at}hsc.usc.edu

Enamel biomineralization is a complex process that involves interactions between extracellular matrix proteins. To identify proteins interacting with tuftelin, a potential nucleator of enamel crystallites, the yeast two-hybrid system was applied to a mouse tooth expression library and a tuftelin-interacting protein (TIP) was isolated for further characterization. Polyclonal antibodies were prepared against two recombinant variants of this protein. Both antibodies identified a major protein product in tooth organs at 39 kDa and this protein has been called TIP39. Northern analysis showed TIP39 messenger RNA in multiple organs, a pattern similar to that of tuftelin messenger RNA. In situ hybridization of mandibles of one day old mice detected TIP39 RNA in secretory ameloblasts and odontoblasts. Immunolocalization of TIP39 and tuftelin in cultured ameloblast-like cells showed that these two proteins colocalize. Within the developing tooth organ, immunolocalization of TIP39 and tuftelin was to the apical pole of secretory ameloblasts (Tomes' processes) and to the newly secreted extracellular enamel matrix. TIP39 amino-acid sequence appears to be highly conserved with similarities to proteins in species as diverse as yeast and primates. Available sequence data and the findings reported here suggest a role for TIP39 in the secretory pathway of extracellular proteins.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
M.L. Paine, M.L. Snead, H.J. Wang, N. Abuladze, A. Pushkin, W. Liu, L.Y. Kao, S.M. Wall, Y.-H. Kim, and I. Kurtz
Role of NBCe1 and AE2 in Secretory Ameloblasts
J. Dent. Res., April 1, 2008; 87(4): 391 - 395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. Paine, W. Luo, H.-J. Wang, P. Bringas Jr., A. Y. W. Ngan, V. G. Miklus, D.-H. Zhu, M. MacDougall, S. N. White, and M. L. Snead
Dentin Sialoprotein and Dentin Phosphoprotein Overexpression during Amelogenesis
J. Biol. Chem., September 9, 2005; 280(36): 31991 - 31998.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
R. L. Slayton, M.E. Cooper, and M.L. Marazita
Tuftelin, Mutans Streptococci, and Dental Caries Susceptibility
J. Dent. Res., August 1, 2005; 84(8): 711 - 714.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. Paine, H.-J. Wang, W. Luo, P. H. Krebsbach, and M. L. Snead
A Transgenic Animal Model Resembling Amelogenesis Imperfecta Related to Ameloblastin Overexpression
J. Biol. Chem., May 23, 2003; 278(21): 19447 - 19452.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.