| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 15, 2000
Biological Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ
Corresponding Author: b.berks{at}uea.ac.uk
The Escherichia coli Tat system mediates Sec-independent export of protein precursors bearing twin-arginine signal peptides. Genes known to be involved in this process include tatA, tatB and tatC which form an operon with a fourth gene, tatD. The tatD gene product has two homologues in E. coli coded by the unlinked ycfH and yjjV genes. An E. coli strain with in-frame chromosomal deletions in all three of tatD, ycfH and yjjV exhibits no significant defect in the cellular location of five cofactor-containing enzymes that are synthesised with twin arginine signal peptides. Neither these mutations, nor overproduction of the TatD protein, cause any discernable effect on the export kinetics of an additional E. coli Tat pathway substrate. It is concluded that proteins of the TatD family have no obligate involvement in protein export by the Tat system. TatD is shown to be a cytoplasmic protein that binds divalent metal cations and that exhibits magnesium-dependent nuclease activity. Features of the tatA operon that may control TatD expression are discussed.
J. Biol. Chem, 10.1074/jbc.M000800200
Submitted on February 2, 2000
Revised on March 10, 2000
Accepted on March 10, 2000
TatD is a cytoplasmic protein with DNAse activity.No requirement for TatD-family proteins in Sec-independent protein export
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. T. Ulijasz, G. Cornilescu, D. von Stetten, S. Kaminski, M. A. Mroginski, J. Zhang, D. Bhaya, P. Hildebrandt, and R. D. Vierstra Characterization of Two Thermostable Cyanobacterial Phytochromes Reveals Global Movements in the Chromophore-binding Domain during Photoconversion J. Biol. Chem., July 25, 2008; 283(30): 21251 - 21266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. L. Barrett, R. Freudl, and C. Robinson Twin Arginine Translocation (Tat)-dependent Export in the Apparent Absence of TatABC or TatA Complexes Using Modified Escherichia coli TatA Subunits That Substitute for TatB J. Biol. Chem., December 14, 2007; 282(50): 36206 - 36213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Richter, U. Lindenstrauss, C. Lucke, R. Bayliss, and T. Bruser Functional Tat Transport of Unstructured, Small, Hydrophilic Proteins J. Biol. Chem., November 16, 2007; 282(46): 33257 - 33264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. K. Bageshwar and S. M. Musser Two electrical potential dependent steps are required for transport by the Escherichia coli Tat machinery J. Cell Biol., October 8, 2007; 179(1): 87 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ren, X. Wang, S. Hao, H. Hu, and C.-c. Wang Translocation of {alpha}-Synuclein Expressed in Escherichia coli J. Bacteriol., April 1, 2007; 189(7): 2777 - 2786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kreutzenbeck, C. Kroger, F. Lausberg, N. Blaudeck, G. A. Sprenger, and R. Freudl Escherichia coli Twin Arginine (Tat) Mutant Translocases Possessing Relaxed Signal Peptide Recognition Specificities J. Biol. Chem., March 16, 2007; 282(11): 7903 - 7911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Stevenson, K. Strisovsky, K. M. Clemmer, S. Bhatt, M. Freeman, and P. N. Rather Rhomboid protease AarA mediates quorum-sensing in Providencia stuartii by activating TatA of the twin-arginine translocase PNAS, January 16, 2007; 104(3): 1003 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Lindenstrauss and T. Bruser Conservation and Variation between Rhodobacter capsulatus and Escherichia coli Tat Systems J. Bacteriol., November 15, 2006; 188(22): 7807 - 7814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Lee, G. L. Orriss, G. Buchanan, N. P. Greene, P. J. Bond, C. Punginelli, R. L. Jack, M. S. P. Sansom, B. C. Berks, and T. Palmer Cysteine-scanning Mutagenesis and Disulfide Mapping Studies of the Conserved Domain of the Twin-arginine Translocase TatB Component J. Biol. Chem., November 10, 2006; 281(45): 34072 - 34085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Saint-Joanis, C. Demangel, M. Jackson, P. Brodin, L. Marsollier, H. Boshoff, and S. T. Cole Inactivation of Rv2525c, a Substrate of the Twin Arginine Translocation (Tat) System of Mycobacterium tuberculosis, Increases {beta}-Lactam Susceptibility and Virulence. J. Bacteriol., September 1, 2006; 188(18): 6669 - 6679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sturm, A. Schierhorn, U. Lindenstrauss, H. Lilie, and T. Bruser YcdB from Escherichia coli Reveals a Novel Class of Tat-dependently Translocated Hemoproteins J. Biol. Chem., May 19, 2006; 281(20): 13972 - 13978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Mullineaux, A. Nenninger, N. Ray, and C. Robinson Diffusion of green fluorescent protein in three cell environments in Escherichia coli. J. Bacteriol., May 1, 2006; 188(10): 3442 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lavander, S. K. Ericsson, J. E. Broms, and A. Forsberg The Twin Arginine Translocation System Is Essential for Virulence of Yersinia pseudotuberculosis Infect. Immun., March 1, 2006; 74(3): 1768 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-N. Chaperon Construction and Complementation of In-Frame Deletions of the Essential Escherichia coli Thymidylate Kinase Gene Appl. Envir. Microbiol., February 1, 2006; 72(2): 1288 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Bronstein, M. Marrichi, S. Cartinhour, D. J. Schneider, and M. P. DeLisa Identification of a Twin-Arginine Translocation System in Pseudomonas syringae pv. tomato DC3000 and Its Contribution to Pathogenicity and Fitness J. Bacteriol., December 15, 2005; 187(24): 8450 - 8461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Gohlke, L. Pullan, C. A. McDevitt, I. Porcelli, E. de Leeuw, T. Palmer, H. R. Saibil, and B. C. Berks The TatA component of the twin-arginine protein transport system forms channel complexes of variable diameter PNAS, July 26, 2005; 102(30): 10482 - 10486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ray, A. Nenninger, C. W. Mullineaux, and C. Robinson Location and Mobility of Twin Arginine Translocase Subunits in the Escherichia coli Plasma Membrane J. Biol. Chem., May 6, 2005; 280(18): 17961 - 17968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qiu, J.-H. Yoon, and B. Shen Search for Apoptotic Nucleases in Yeast: ROLE OF Tat-D NUCLEASE IN APOPTOTIC DNA DEGRADATION J. Biol. Chem., April 15, 2005; 280(15): 15370 - 15379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Blaudeck, P. Kreutzenbeck, M. Muller, G. A. Sprenger, and R. Freudl Isolation and Characterization of Bifunctional Escherichia coli TatA Mutant Proteins That Allow Efficient Tat-dependent Protein Translocation in the Absence of TatB J. Biol. Chem., February 4, 2005; 280(5): 3426 - 3432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kimata and M. Yanagida Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis J. Cell Sci., May 1, 2004; 117(11): 2283 - 2293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gouffi, F. Gerard, C.-L. Santini, and L.-F. Wu Dual Topology of the Escherichia coli TatA Protein J. Biol. Chem., March 19, 2004; 279(12): 11608 - 11615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. DeLisa, P. Lee, T. Palmer, and G. Georgiou Phage Shock Protein PspA of Escherichia coli Relieves Saturation of Protein Export via the Tat Pathway J. Bacteriol., January 15, 2004; 186(2): 366 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Papish, C. L. Ladner, and R. J. Turner The Twin-arginine Leader-binding Protein, DmsD, Interacts with the TatB and TatC Subunits of the Escherichia coli Twin-arginine Translocase J. Biol. Chem., August 29, 2003; 278(35): 32501 - 32506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Blaudeck, P. Kreutzenbeck, R. Freudl, and G. A. Sprenger Genetic Analysis of Pathway Specificity during Posttranslational Protein Translocation across the Escherichia coli Plasma Membrane J. Bacteriol., May 1, 2003; 185(9): 2811 - 2819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Palmer and B. C. Berks Moving folded proteins across the bacterial cell membrane Microbiology, March 1, 2003; 149(3): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ding and P. J. Christie Agrobacterium tumefaciens Twin-Arginine-Dependent Translocation Is Important for Virulence, Flagellation, and Chemotaxis but Not Type IV Secretion J. Bacteriol., February 1, 2003; 185(3): 760 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alami, D. Trescher, L.-F. Wu, and M. Muller Separate Analysis of Twin-arginine Translocation (Tat)-specific Membrane Binding and Translocation in Escherichia coli J. Biol. Chem., May 31, 2002; 277(23): 20499 - 20503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. H. Allen, C. M. L. Barrett, N. Ray, and C. Robinson Essential Cytoplasmic Domains in the Escherichia coli TatC Protein J. Biol. Chem., March 15, 2002; 277(12): 10362 - 10366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pop, U. Martin, C. Abel, and J. P. Muller The Twin-arginine Signal Peptide of PhoD and the TatAd/Cd Proteins of Bacillus subtilis Form an Autonomous Tat Translocation System J. Biol. Chem., January 25, 2002; 277(5): 3268 - 3273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Heikkilä, U. Honisch, P. Wunsch, and W. G. Zumft Role of the Tat Transport System in Nitrous Oxide Reductase Translocation and Cytochrome cd1 Biosynthesis in Pseudomonas stutzeri J. Bacteriol., March 1, 2001; 183(5): 1663 - 1671. [Abstract] [Full Text]
|
|
|
|
|
|
R. L. Jack, F. Sargent, B. C. Berks, G. Sawers, and T. Palmer Constitutive Expression of Escherichia coli tat Genes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth J. Bacteriol., March 1, 2001; 183(5): 1801 - 1804. [Abstract] [Full Text]
|
|
|
|
|
|
N. R. Stanley, K. Findlay, B. C. Berks, and T. Palmer Escherichia coli Strains Blocked in Tat-Dependent Protein Export Exhibit Pleiotropic Defects in the Cell Envelope J. Bacteriol., January 1, 2001; 183(1): 139 - 144. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. H. Jongbloed, U. Martin, H. Antelmann, M. Hecker, H. Tjalsma, G. Venema, S. Bron, J. M. van Dijl, and J. Muller TatC Is a Specificity Determinant for Protein Secretion via the Twin-arginine Translocation Pathway J. Biol. Chem., December 22, 2000; 275(52): 41350 - 41357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sambasivarao, H. A. Dawson, G. Zhang, G. Shaw, J. Hu, and J. H. Weiner Investigation of Escherichia coli Dimethyl Sulfoxide Reductase Assembly and Processing in Strains Defective for the sec-Independent Protein Translocation System Membrane Targeting and Translocation J. Biol. Chem., June 1, 2001; 276(23): 20167 - 20174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bolhuis, J. E. Mathers, J. D. Thomas, C. M. L. Barrett, and C. Robinson TatB and TatC Form a Functional and Structural Unit of the Twin-arginine Translocase from Escherichia coli J. Biol. Chem., June 1, 2001; 276(23): 20213 - 20219. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
