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Papers In Press, published online ahead of print October 12, 2000
Biologie et Pathologie digestive, INSERM U 531, Toulouse 31403
Corresponding Author: Francois.Clemente{at}rangueil.inserm.fr
Abstract The HMW FGF-2 isoform of 210 amino acids initiated at a CUG start codon possesses a nuclear localization sequence and is not secreted. In contrast the LMW isoform of 155 amino acids initiated at the AUG start codon can be secreted and activates the cell surface FGF receptors. The two isoforms possess different biological properties, however little is known about the intracrine regulatory mechanisms involved in the biological effects of the HMW FGF-2 isoform. Using pancreatic cells stably transfected with cDNAs leading to the expression of either the HMW FGF-2 (A3 cells) or the LMW form (A5 cells), we provide evidence that the two FGF-2 isoforms differentially modulate PKC levels. The LMW FGF-2 upregulated the PKC epsilon levels by 1.6 fold, by contrast the HMW isoform downregulated the level of this PKC isotype by about 3 fold and increased the amount of PKC delta by 1.7 fold. PKC mRNAs were also modified suggesting that PKC expression was regulated at a pre-translational level. Additionally, expression of different levels of the HMW FGF-2 with an inducible expression system confirmed the role of this isoform on PKC delta and epsilon expressions. Increased activation of ERK-1 and -2 was also observed in cells expressing the HMW FGF-2. By using different PKC inhibitors and a dominant negative PKC delta, it was found that ERK activation was PKC delta-dependent. These data indicate that expression of HMW FGF-2 can modify PKC levels by acting at the intracellular level and that the over-expression of PKC delta induces ERK-1/2 activation. The expression of a dominant negative FGFR1 did not reduce ERK-1/2 activation by the HMW FGF-2 suggesting that ERK activation does not require FGFR activity. The signaling cascade downstream of ERK might be involved in the known mitogenic effect exerted by this FGF-2 isoform.
J. Biol. Chem, 10.1074/jbc.M001184200
Submitted on February 11, 2000
Revised on October 12, 2000
Accepted on October 12, 2000
Expression of the HMW FGF-2 Isoform of 210 Isoform of 210 Amino Acids is Associated with Modulation of Protein Kinase C
and 
and ERK Activation
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