JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 14, 2000
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
275/29/22166    most recent
M001746200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, A. K
Right arrow Articles by Blackwell, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, A. K
Right arrow Articles by Blackwell, T. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print April 6, 2000
J. Biol. Chem, 10.1074/jbc.M001746200
Submitted on March 2, 2000
Accepted on April 5, 2000

A conserved transcription motif suggesting functional parallels between C. elegans SKN-1 and Cap'n'Collar-related bZIP proteins

Amy K Walker, Raymond See, Ceri Batchelder, Thip Kophengnavong, J. Timothy Gronniger, Yang Shi, and T. Keith Blackwell

Center for Blood Research, Harvard Medical School, Boston, MA 02115

Corresponding Author: blackwell{at}cbr.med.harvard.edu

In C. elegans, the predicted transcription factor SKN-1 is required for embryonic endodermal and mesodermal specification, and for maintaining differentiated intestinal cells postembryonically. The SKN-1 DNA binding region is related to the Cap'n'Collar (CNC) family of basic leucine zipper (bZIP) proteins, but uniquely, SKN-1 binds DNA as a monomer. CNC proteins are absent in C. elegans, however, and their involvement in the endoderm and mesoderm suggests some functional parallels to SKN-1. Using a cell culture assay, we show that SKN-1 induces transcription and contains three potent activation domains. The functional core of one domain is a short motif, the DIDLID element, which is highly conserved in a subgroup of vertebrate CNC proteins. The DIDLID element is important for SKN-1-driven transcription, suggesting likely significance in other CNC proteins. SKN-1 binds to and activates transcription through the p300/CBP coactivator, supporting the genetic prediction that SKN-1 recruits the C. elegans p300/CBP ortholog, CBP-1. The DIDLID element appears to act independently of p300/CBP, however, suggesting a distinct conserved target. The evolutionarily preservation of the DIDLID transcriptional element supports the model that SKN-1 and some CNC proteins interact with analogous co-factors, and may have preserved some similar functions despite divergent DNA binding domains.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
From the Cover: Nrf2 mediates cancer protection but not prolongevity induced by caloric restriction
PNAS, February 19, 2008; 105(7): 2325 - 2330.


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. H. An, K. Vranas, M. Lucke, H. Inoue, N. Hisamoto, K. Matsumoto, and T. K. Blackwell
Regulation of the Caenorhabditis elegans oxidative stress defense protein SKN-1 by glycogen synthase kinase-3
PNAS, November 8, 2005; 102(45): 16275 - 16280.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
B. Arca, F. Lombardo, J. G. Valenzuela, I. M. B. Francischetti, O. Marinotti, M. Coluzzi, and J. M. C. Ribeiro
An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae
J. Exp. Biol., October 15, 2005; 208(20): 3971 - 3986.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. Goszczynski and J. D. McGhee
Reevaluation of the Role of the med-1 and med-2 Genes in Specifying the Caenorhabditis elegans Endoderm
Genetics, October 1, 2005; 171(2): 545 - 555.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. Inoue, N. Hisamoto, J. H. An, R. P. Oliveira, E. Nishida, T. K. Blackwell, and K. Matsumoto
The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response
Genes & Dev., October 1, 2005; 19(19): 2278 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. A. Hanover, M. E. Forsythe, P. T. Hennessey, T. M. Brodigan, D. C. Love, G. Ashwell, and M. Krause
A Caenorhabditis elegans model of insulin resistance: Altered macronutrient storage and dauer formation in an OGT-1 knockout
PNAS, August 9, 2005; 102(32): 11266 - 11271.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. McMahon, N. Thomas, K. Itoh, M. Yamamoto, and J. D. Hayes
Redox-regulated Turnover of Nrf2 Is Determined by at Least Two Separate Protein Domains, the Redox-sensitive Neh2 Degron and the Redox-insensitive Neh6 Degron
J. Biol. Chem., July 23, 2004; 279(30): 31556 - 31567.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. H. An and T. K. Blackwell
SKN-1 links C. elegans mesendodermal specification to a conserved oxidative stress response
Genes & Dev., August 1, 2003; 17(15): 1882 - 1893.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.