| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print April 6, 2000
Dana-Farber Cancer Institute, Boston, M.A. 02115
Corresponding Author: rakesh_datta{at}dfci.harvard.edu
Protein kinase C (PKC)
J. Biol. Chem, 10.1074/jbc.M002266200
Submitted on March 17, 2000
Accepted on April 3, 2000
Proteolytic Cleavage and Activation of Protein Kinase C
by Caspase-3 in Apoptotoic Response of cells to 1-
-D-Arabinofuranosylcytosine and other Genotoxic Agents
is a novel member of the PKC family that differs from the other isozymes in structural and biochemical properties. The precise function of PKCm is not known. The present studies demonstrate that PKCm is cleaved during apoptosis induced by 1-
-D-arabinofuranosylcytosine (ara-C) and other genotoxic agents. PKC cleavage is blocked in cells that overexpress the anti-apoptotic Bcl-xL protein or the baculovirus p35 protein. Our results demonstrate that PKC is cleaved by caspase-3 at the CQND378S site. Cleavage of PKC is associated with release of the catalytic domain and activation of its kinase function. We also show that, unlike the cleaved fragments of PKC
and
, overexpression of the PKC catalytic domain is not lethal. Cells stably expressing the catalytic fragment of PKC, however, are more sensitive to apoptosis induced by genotoxic stress. In addition, expression of caspase-resistant PKC mutant partially inhibits DNA damage-induced apoptosis. These findings demonstrate that PKC is cleaved by caspase-3 and that expression of the catalytic domain sensitizes cells to the cytotoxic effects of ara-C and other anti-cancer agents.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Avkiran, A. J. Rowland, F. Cuello, and R. S. Haworth Protein Kinase D in the Cardiovascular System: Emerging Roles in Health and Disease Circ. Res., February 1, 2008; 102(2): 157 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Johannessen, M. P. Delghandi, A. Rykx, M. Dragset, J. R. Vandenheede, J. Van Lint, and U. Moens Protein Kinase D Induces Transcription through Direct Phosphorylation of the cAMP-response Element-binding Protein J. Biol. Chem., May 18, 2007; 282(20): 14777 - 14787. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Neuzil, M. Tomasetti, Y. Zhao, L.-F. Dong, M. Birringer, X.-F. Wang, P. Low, K. Wu, B. A. Salvatore, and S. J. Ralph Vitamin E Analogs, a Novel Group of "Mitocans," as Anticancer Agents: The Importance of Being Redox-Silent Mol. Pharmacol., May 1, 2007; 71(5): 1185 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Renault-Mihara, F. Beuvon, X. Iturrioz, B. Canton, S. De Bouard, N. Leonard, S. Mouhamad, A. Sharif, J. W. Ramos, M.-P. Junier, et al. Phosphoprotein Enriched in Astrocytes-15 kDa Expression Inhibits Astrocyte Migration by a Protein Kinase C{delta}-dependent Mechanism Mol. Biol. Cell, December 1, 2006; 17(12): 5141 - 5152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Thottassery, L. Westbrook, H. Someya, and W. B. Parker c-Abl-independent p73 stabilization during gemcitabine- or 4'-thio-{beta}-D-arabinofuranosylcytosine-induced apoptosis in wild-type and p53-null colorectal cancer cells. Mol. Cancer Ther., February 1, 2006; 5(2): 400 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wong and Z.-G. Jin Protein Kinase C-dependent Protein Kinase D Activation Modulates ERK Signal Pathway and Endothelial Cell Proliferation by Vascular Endothelial Growth Factor J. Biol. Chem., September 30, 2005; 280(39): 33262 - 33269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Okhrimenko, W. Lu, C. Xiang, N. Hamburger, G. Kazimirsky, and C. Brodie Protein Kinase C-{varepsilon} Regulates the Apoptosis and Survival of Glioma Cells Cancer Res., August 15, 2005; 65(16): 7301 - 7309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rozengurt, O. Rey, and R. T. Waldron Protein Kinase D Signaling J. Biol. Chem., April 8, 2005; 280(14): 13205 - 13208. [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, G. Lu, and Q. J. Wang Protein Kinase C-Independent Effects of Protein Kinase D3 in Glucose Transport in L6 Myotubes Mol. Pharmacol., January 1, 2005; 67(1): 152 - 162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tan, X. Xu, M. Ohba, and M.-Z. Cui Angiotensin II-Induced Protein Kinase D Activation Is Regulated by Protein Kinase C{delta} and Mediated via the Angiotensin II Type 1 Receptor in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., December 1, 2004; 24(12): 2271 - 2276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Kennett, J. D. Roberts, and K. Olden Requirement of Protein Kinase C{micro} Activation and Calpain-mediated Proteolysis for Arachidonic Acid-stimulated Adhesion of MDA-MB-435 Human Mammary Carcinoma Cells to Collagen Type IV J. Biol. Chem., January 30, 2004; 279(5): 3300 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lemonnier, C. Ghayor, J. Guicheux, and J. Caverzasio Protein Kinase C-independent Activation of Protein Kinase D Is Involved in BMP-2-induced Activation of Stress Mitogen-activated Protein Kinases JNK and p38 and Osteoblastic Cell Differentiation J. Biol. Chem., January 2, 2004; 279(1): 259 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Storz, H. Doppler, F.-J. Johannes, and A. Toker Tyrosine Phosphorylation of Protein Kinase D in the Pleckstrin Homology Domain Leads to Activation J. Biol. Chem., May 9, 2003; 278(20): 17969 - 17976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Smith and J. B. Smith Lack of Constitutive Activity of the Free Kinase Domain of Protein Kinase C zeta . DEPENDENCE ON TRANSPHOSPHORYLATION OF THE ACTIVATION LOOP J. Biol. Chem., November 22, 2002; 277(48): 45866 - 45873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Basu, D. Lu, B. Sun, A. N. Moor, G. R. Akkaraju, and J. Huang Proteolytic Activation of Protein Kinase C-epsilon by Caspase-mediated Processing and Transduction of Antiapoptotic Signals J. Biol. Chem., October 25, 2002; 277(44): 41850 - 41856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ren, R. Datta, H. Shioya, Y. Li, E. Oki, V. Biedermann, A. Bharti, and D. Kufe p73beta Is Regulated by Protein Kinase Cdelta Catalytic Fragment Generated in the Apoptotic Response to DNA Damage J. Biol. Chem., September 6, 2002; 277(37): 33758 - 33765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Johnson, D. Lu, J. Huang, and A. Basu Regulation of p53 Stabilization by DNA Damage and Protein Kinase C Mol. Cancer Ther., August 1, 2002; 1(10): 861 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hartmann, L. Johnk, G. Kitange, Y. Wu, L. K. Ashworth, R. B. Jenkins, and D. N. Louis Transcript Map of the 3.7-Mb D19S112-D19S246 Candidate Tumor Suppressor Region on the Long Arm of Chromosome 19 Cancer Res., July 15, 2002; 62(14): 4100 - 4108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zrachia, M. Dobroslav, M. Blass, G. Kazimirsky, I. Kronfeld, P. M. Blumberg, D. Kobiler, S. Lustig, and C. Brodie Infection of Glioma Cells with Sindbis Virus Induces Selective Activation and Tyrosine Phosphorylation of Protein Kinase C delta . IMPLICATIONS FOR SINDBIS VIRUS-INDUCED APOPTOSIS J. Biol. Chem., June 21, 2002; 277(26): 23693 - 23701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Paszty, A. K. Verma, R. Padanyi, A. G. Filoteo, J. T. Penniston, and A. Enyedi Plasma Membrane Ca2+ATPase Isoform 4b Is Cleaved and Activated by Caspase-3 during the Early Phase of Apoptosis J. Biol. Chem., February 22, 2002; 277(9): 6822 - 6829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Brose and C. Rosenmund Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters J. Cell Sci., January 12, 2002; 115(23): 4399 - 4411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Blass, I. Kronfeld, G. Kazimirsky, P. M. Blumberg, and C. Brodie Tyrosine Phosphorylation of Protein Kinase C{delta} Is Essential for Its Apoptotic Effect in Response to Etoposide Mol. Cell. Biol., January 1, 2002; 22(1): 182 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sturany, J. Van Lint, F. Muller, M. Wilda, H. Hameister, M. Hocker, A. Brey, U. Gern, J. Vandenheede, T. Gress, et al. Molecular Cloning and Characterization of the Human Protein Kinase D2. A NOVEL MEMBER OF THE PROTEIN KINASE D FAMILY OF SERINE THREONINE KINASES J. Biol. Chem., January 26, 2001; 276(5): 3310 - 3318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Matassa, L. Carpenter, T. J. Biden, M. J. Humphries, and M. E. Reyland PKCdelta Is Required for Mitochondrial-dependent Apoptosis in Salivary Epithelial Cells J. Biol. Chem., August 3, 2001; 276(32): 29719 - 29728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Waldron, O. Rey, T. Iglesias, T. Tugal, D. Cantrell, and E. Rozengurt Activation Loop Ser744 and Ser748 in Protein Kinase D Are Transphosphorylated in Vivo J. Biol. Chem., August 24, 2001; 276(35): 32606 - 32615. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
