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M002996200v1
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Papers In Press, published online ahead of print May 26, 2000
J. Biol. Chem, 10.1074/jbc.M002996200
Submitted on April 9, 2000
Revised on May 16, 2000
Accepted on May 26, 2000

Fibrillarin genes encode both a conserved nucleolar protein and a novel snoRNA involved in rRNA methylation in Arabidopsis thaliana

Frédy Barneche, Florence Steinmetz, and Manuel Echeverria

Laboratoire Génome et Développement des Plantes - UMR CNRS 5096, Université de Perpignan, Perpignan 66860

Corresponding Author: manuel{at}univ-perp.fr

Fibrillarin is a key nucleolar protein in eukaryotes that associates with box C/D snoRNAs directing 2'-0-ribose methylation of the rRNA. In this study we describe two genes in Arabidopsis thaliana, AtFib1 and AtFib2, encoding nearly identical proteins conserved with eukaryotic fibrillarins. We demonstrate that AtFib1 and AtFib2 proteins are functional homologues of the yeast Nop1p (fibrillarin) and can rescue a yeast NOP1-null mutant strain. Surprisingly, for the first time in plants, we identified two isoforms of a novel box C/D snoRNA, U60.1f and U60.2f, nested in the fifth intron of AtFib1 and AtFib2. Interestingly after gene duplication the host intronic sequences completely diverged but the snoRNA was conserved, even in other crucifer fibrillarin genes. We show that the U60f snoRNAs accumulate in seedlings and that their targeted residue on the 25S rRNA is methylated. Our data reveal that the three modes of expression of snoRNAs, single, polycistronic and intronic exist in plants, and suggest that the mechanisms directing rRNA methylation, dependent on fibrillarin and box C/D snoRNAs, are evolutionarily conserved in plants.


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