JBC Biosymposia, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 10, 2000
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
275/46/35684    most recent
M004315200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frit, P.
Right arrow Articles by Calsou, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frit, P.
Right arrow Articles by Calsou, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print August 16, 2000
J. Biol. Chem, 10.1074/jbc.M004315200
Submitted on May 19, 2000
Revised on July 7, 2000
Accepted on August 16, 2000

Ku entry into DNA inhibits inward DNA transactions in vitro

Philippe Frit, Doriane Arzel, Ruo-Ya Li, Bernard Salles, and Patrick Calsou

Institut de Pharmacologie et de Biologie Structurale, CNRS, UMR5089, Toulouse, Cedex 4 31077

Corresponding Author: calsou{at}ipbs.fr

Association of the DNA-end binding Ku70/Ku80 heterodimer with the 460 kDa serine/threonine kinase catalytic subunit forms the DNA-dependent protein kinase (DNA-PK) which is required for double-strand break repair by non-homologous recombination in mammalian cells. Recently, we have proposed a model in which the kinase activity is required for translocation of the DNA-end binding subunit Ku along the DNA helix when DNA-PK assembles on DNA-ends. Here, we have questioned the consequences of Ku entry into DNA on local DNA processes by using human nuclear cell extracts incubated in the presence of linearized plasmid DNA. As two model processes, we have chosen nucleotide excision repair (NER) of UVC DNA-lesions and transcription from viral promoters. We show that although NER efficiency is strongly reduced on linear DNA, it can be fully restored in the presence of DNA-PK inhibitors. Simultaneously, the amount of NER proteins bound to the UVC-damaged linear DNA is increased and the amount of Ku bound to the same DNA molecules is decreased. Similarly, the poor transcription efficiency exhibited by viral promoters on linear DNA is enhanced in the presence of DNA-PK inhibitor concentrations that prevent Ku entry into the DNA substrate molecule. The present results show that DNA-PK catalytic activity can regulate DNA transactions including transcription in the vicinity of double-strand breaks by controlling Ku entry into DNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. P. Diggle, J. Bentley, M. A. Knowles, and A. E. Kiltie
Inhibition of double-strand break non-homologous end-joining by cisplatin adducts in human cell extracts
Nucleic Acids Res., May 4, 2005; 33(8): 2531 - 2539.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Drouet, C. Delteil, J. Lefrancois, P. Concannon, B. Salles, and P. Calsou
DNA-dependent Protein Kinase and XRCC4-DNA Ligase IV Mobilization in the Cell in Response to DNA Double Strand Breaks
J. Biol. Chem., February 25, 2005; 280(8): 7060 - 7069.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Onclercq-Delic, P. Calsou, C. Delteil, B. Salles, D. Papadopoulo, and M. Amor-Gueret
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing
Nucleic Acids Res., November 1, 2003; 31(21): 6272 - 6282.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.