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Papers In Press, published online ahead of print July 19, 2000
Department of Molecular Biology, Biomolecular Engineering Resaerch Institute, Suita, Osaka 565-0874
Corresponding Author: ishino{at}beri.co.jp
RecA and Rad51 proteins are essential for homologous recombination in Bacteria and Eukarya, respectively. Homologous proteins, called RadA, have been described for Archaea. Here we present the characterization of two RecA/Rad51 family proteins, RadA and RadB, from Pyrococcus furiosus. The radA and radB genes were not induced by DNA damage resulting from exposure of the cells to gamma-, UV-irradiation and heat shock, suggesting that they might be constitutively expressed in this hyperthermophile. RadA had DNA-dependent ATPase, D-loop formation, and strand exchange activities. In contrast, RadB had a very weak ATPase activity that is not stimulated by DNA. This protein had a strong binding affinity for DNA, but little strand-exchange activity could be detected. A direct interaction between RadA and RadB was detected by an immunoprecipitation assay. Moreover, RadB, but not RadA, coprecipitated with Hjc, a Holliday junction resolvase found in P. furiosus, in the absence of ATP. This interaction was suppressed in the presence of ATP. The Holliday junction cleavage activity of Hjc was inhibited by RadB in the absence, but not in the presence, of ATP. These results suggest that RadB has important roles in homologous recombination in Archaea, and may regulate the cleavage reactions of the branch-structured DNA.
J. Biol. Chem, 10.1074/jbc.M004557200
Submitted on May 26, 2000
Revised on June 30, 2000
Accepted on July 19, 2000
Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus
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