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Papers In Press, published online ahead of print October 19, 2000
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194-8533
Corresponding Author: ksasaki{at}kyowa.co.jp
We have isolated three types of cDNAs encoding novel b1,3-N-acetylglucosaminyltransferases (designated b3Gn-T2, -T3, and -T4) from human gastric mucosa and neuroblastoma cell line SK-N-MC. These enzymes were predicted to be type II transmembrane proteins of 397, 372, and 378 amino acid residues, respectively. They shared motifs conserved among b1,3-galactosyltransferase family and a b1,3-N-acetylglucosaminyltransferase (designated b3Gn-T1), but showed no structural similarity to another type of b1,3-N-acetylglucosaminyltransferase (iGnT). Each of the enzymes expressed by insect cells as a secreted protein fused with FLAG peptide showed b1,3-N-acetylglucosaminyltransferase activity toward type II oligosaccharides, but not b1,3-galactosyltransferase activity. These enzymes exhibited different substrate specificity. Transfection of Namalwa KJM-1 cells with b3Gn-T2, -T3, or -T4 cDNA led to increase of poly-N-acetyllactosamines recognized by an anti i-antigen antibody or specific lectins. The expression profiles of these b3Gn-Ts were different among 35 human tissues. b3Gn-T2 was ubiquitously expressed, while the expression of b3Gn-T3 and -T4 was relatively restricted. b3Gn-T2 was expressed at colon, jejunum, stomach, esophagus, placenta, and trachea. b3Gn-T4 was mainly expressed at brain. These results have revealed that several b1,3-N-acetylglucosaminyltransferases form a family with structural similarity to the b1,3-galactosyltransferase family. Considering difference in substrate specificity and distribution, each b1,3-N-acetylglucosaminyltransferase may play different roles.
J. Biol. Chem, 10.1074/jbc.M004800200
Submitted on June 2, 2000
Revised on October 19, 2000
Accepted on October 19, 2000
Identification and characterization of three novel beta1,3-N-acetylglucosaminyltransferases structurally related to the beta1,3-galactosyltransferase family
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