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Papers In Press, published online ahead of print December 5, 2000
Lab of Molecular Genetics, NIEHS, Research Triangle Park, NC 27709-2233
Corresponding Author: worth{at}niehs.nih.gov
This study examines the interaction between Escherichia coli MutS, L and E. coli RuvAB during E. coli RecA-promoted strand exchange. RuvAB is a branch migration complex that stimulates heterologous strand exchange. Previous studies indicate that RuvAB increases the rate at which heteroduplex product is formed by RecA, that RuvA and RuvB are required for this stimulation, and that RuvAB does not stimulate homologous strand exchange. This study indicates that MutS, L inhibits the formation of full-length heteroduplex between M13-fd DNA in the presence of RuvAB, such that less than 2% of the linear substrate is converted to product. Inhibition depends on the time at which MutS, L is added to the reaction and is strongest when MutS, L is added during initiation. The kinetics of the strand exchange reaction suggest that MutS, L directly inhibits RuvAB-dependent branch migration in the absence of RecA. The inhibition requires the formation of base-base mismatches and ATP utilization; no effect on RuvAB-promoted strand exchange is seen if an ATP-deficient mutant of MutS (MutS501) is included in the reaction instead of wild type MutS. These results are consistent with a role for MutS, L in maintaining genomic stability and replication fidelity.
J. Biol. Chem, 10.1074/jbc.M005176200
Submitted on June 14, 2000
Revised on November 1, 2000
Accepted on December 4, 2000
Escherichia coli MutS, L modulate RuvAB-dependent branch migration between diverged DNA
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