| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print September 19, 2000
Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv 69978
Corresponding Author: dascaln{at}post.tau.ac.il
Neuronal voltage-dependent Ca2+ channels (VDCCs) of the N (
J. Biol. Chem, 10.1074/jbc.M005881200
Submitted on July 5, 2000
Accepted on September 19, 2000
Modulation of L-type Ca2+ channels by G{beta
} and calmodulin via interactions with N- and C-termini of alpha1C
1B) and P/Q (1A) type are inhibited by neurotransmitters that activate Gi/o G proteins; a major part of the inhibition is voltage-dependent, relieved by depolarization, and results from a direct binding of G{beta
} subunit of G proteins to the channel. Since cardiac and neuronal L-type (1C) VDCCs are not modulated in this way, they are presumed to lack interaction with G{beta}. However, here we demonstrate that both G{beta} and calmodulin directly bind to cytosolic N and C termini of the 1C subunit. Coexpression of G{beta} reduces the current via the L-type channels. The inhibition depends on the presence of calmodulin, occurs at basal cellular levels of Ca2+, and is eliminated by EGTA. The N- and C-termini of 1C appear to serve as partially independent but interacting inhibitory gates. Deletion of the N-terminus or of the distal half of the C-terminus eliminates the inhibitory effect of G{beta}. Deletion of the N-terminus (which binds calmodulin) profoundly impairs the Ca2+/calmodulin - dependent inactivation. We propose that G{beta} and calmodulin regulate the L-type Ca2+ channel in a concerted manner via a molecular inhibitory scaffold formed by N- and C-termini of 1C.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Morrey, R. Estephan, G. W. Abbott, and R. Levi Cardioprotective Effect of Histamine H3-Receptor Activation: Pivotal Role of G{beta}{gamma}-Dependent Inhibition of Voltage-Operated Ca2+ Channels J. Pharmacol. Exp. Ther., September 1, 2008; 326(3): 871 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. O-Uchi, H. Sasaki, S. Morimoto, Y. Kusakari, H. Shinji, T. Obata, K. Hongo, K. Komukai, and S. Kurihara Interaction of {alpha}1-Adrenoceptor Subtypes With Different G Proteins Induces Opposite Effects on Cardiac L-type Ca2+ Channel Circ. Res., June 6, 2008; 102(11): 1378 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cui, A. C. Meyer, I. Calin-Jageman, J. Neef, F. Haeseleer, T. Moser, and A. Lee Ca2+-binding proteins tune Ca2+-feedback to Cav1.3 channels in mouse auditory hair cells J. Physiol., December 15, 2007; 585(3): 791 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Tippens and A. Lee Caldendrin, a Neuron-specific Modulator of Cav/1.2 (L-type) Ca2+ Channels J. Biol. Chem., March 16, 2007; 282(11): 8464 - 8473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Calin-Jageman, K. Yu, R. A. Hall, L. Mei, and A. Lee Erbin Enhances Voltage-Dependent Facilitation of Cav1.3 Ca2+ Channels through Relief of an Autoinhibitory Domain in the Cav1.3 {alpha}1 Subunit J. Neurosci., February 7, 2007; 27(6): 1374 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Seu and G. S. Pitt Dose-dependent and Isoform-specific Modulation of Ca2+ Channels by RGK GTPases J. Gen. Physiol., November 1, 2006; 128(5): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-N. Yang and P.-O. Berggren The Role of Voltage-Gated Calcium Channels in Pancreatic {beta}-Cell Physiology and Pathophysiology Endocr. Rev., October 1, 2006; 27(6): 621 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-S. Lee, R. Karl, S. Moosmang, P. Lenhardt, N. Klugbauer, F. Hofmann, T. Kleppisch, and A. Welling Calmodulin Kinase II Is Involved in Voltage-dependent Facilitation of the L-type Cav1.2 Calcium Channel: IDENTIFICATION OF THE PHOSPHORYLATION SITES J. Biol. Chem., September 1, 2006; 281(35): 25560 - 25567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kanevsky and N. Dascal Regulation of Maximal Open Probability Is a Separable Function of Cav{beta} Subunit in L-type Ca2+ Channel, Dependent on NH2 Terminus of {alpha}1C (Cav1.2{alpha}) J. Gen. Physiol., June 26, 2006; 128(1): 15 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Gibson, M. C. Lawrence, C. J. Gibson, C. A. Vanderbilt, K. McGlynn, D. Arnette, W. Chen, J. Collins, B. Naziruddin, M. F. Levy, et al. Inhibition of Glucose-Stimulated Activation of Extracellular Signal-Regulated Protein Kinases 1 and 2 by Epinephrine in Pancreatic {beta}-Cells. Diabetes, April 1, 2006; 55(4): 1066 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhou, K. Yu, K. L. McCoy, and A. Lee Molecular Mechanism for Divergent Regulation of Cav1.2 Ca2+ Channels by Calmodulin and Ca2+-binding Protein-1 J. Biol. Chem., August 19, 2005; 280(33): 29612 - 29619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xiong, Q. K. Kleerekoper, R. He, J. A. Putkey, and S. L. Hamilton Sites on Calmodulin That Interact with the C-terminal Tail of Cav1.2 Channel J. Biol. Chem., February 25, 2005; 280(8): 7070 - 7079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-N. Yang and P.-O. Berggren {beta}-Cell CaV channel regulation in physiology and pathophysiology Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E16 - E28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-J. Xu, L.-Y. Hao, A. Kameyama, and M. Kameyama Calmodulin reverses rundown of L-type Ca2+ channels in guinea pig ventricular myocytes Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1717 - C1724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Liao, D. Yu, S. Lu, Z. Tang, M. C. Liang, S. Zeng, W. Lin, and T. W. Soong Smooth Muscle-selective Alternatively Spliced Exon Generates Functional Variation in Cav1.2 Calcium Channels J. Biol. Chem., November 26, 2004; 279(48): 50329 - 50335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lipscombe, T. D. Helton, and W. Xu L-Type Calcium Channels: The Low Down J Neurophysiol, November 1, 2004; 92(5): 2633 - 2641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Z. Tang, M. C. Liang, S. Lu, D. Yu, C. Y. Yu, D. T. Yue, and T. W. Soong Transcript Scanning Reveals Novel and Extensive Splice Variations in Human L-type Voltage-gated Calcium Channel, Cav1.2 {alpha}1 Subunit J. Biol. Chem., October 22, 2004; 279(43): 44335 - 44343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Milovic, B. Steinecker-Frohnwieser, W. Schreibmayer, and L. G. Weigl The Sensitivity of G Protein-activated K+ Channels toward Halothane Is Essentially Determined by the C Terminus J. Biol. Chem., August 13, 2004; 279(33): 34240 - 34249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Gomez, B.-G. Kerfant, G. Vassort, and A. J. Pappano Autonomic regulation of calcium and potassium channels is oppositely modulated by microtubules in cardiac myocytes Am J Physiol Heart Circ Physiol, June 1, 2004; 286(6): H2065 - H2071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Isaev, K. Solt, O. Gurtovaya, J. P. Reeves, and R. Shirokov Modulation of the Voltage Sensor of L-type Ca2+ Channels by Intracellular Ca2+ J. Gen. Physiol., April 26, 2004; 123(5): 555 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. X. Mori, M. G. Erickson, and D. T. Yue Functional Stoichiometry and Local Enrichment of Calmodulin Interacting with Ca2+ Channels Science, April 16, 2004; 304(5669): 432 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Blumenstein, O. P. Maximyuk, N. Lozovaya, N. M. Yatsenko, N. Kanevsky, O. Krishtal, and N. Dascal Intracellular Na+ inhibits voltage-dependent N-type Ca2+ channels by a G protein {beta}{gamma} subunit-dependent mechanism J. Physiol., April 1, 2004; 556(1): 121 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lee, H. Zhou, T. Scheuer, and W. A. Catterall Molecular determinants of Ca2+/calmodulin-dependent regulation of Cav2.1 channels PNAS, December 23, 2003; 100(26): 16059 - 16064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ivanina, I. Rishal, D. Varon, C. Mullner, B. Frohnwieser-Steinecke, W. Schreibmayer, C. W. Dessauer, and N. Dascal Mapping the G{beta}{gamma}-binding Sites in GIRK1 and GIRK2 Subunits of the G Protein-activated K+ Channel J. Biol. Chem., August 1, 2003; 278(31): 29174 - 29183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vigh, E. Solessio, C. W. Morgans, and E. M. Lasater Ionic Mechanisms Mediating Oscillatory Membrane Potentials in Wide-Field Retinal Amacrine Cells J Neurophysiol, July 1, 2003; 90(1): 431 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kobrinsky, E. Schwartz, D. R. Abernethy, and N. M. Soldatov Voltage-gated Mobility of the Ca2+ Channel Cytoplasmic Tails and Its Regulatory Role J. Biol. Chem., February 7, 2003; 278(7): 5021 - 5028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rishal, T. Keren-Raifman, D. Yakubovich, T. Ivanina, C. W. Dessauer, V. Z. Slepak, and N. Dascal Na+ Promotes the Dissociation between Galpha GDP and Gbeta gamma , Activating G Protein-gated K+ Channels J. Biol. Chem., January 31, 2003; 278(6): 3840 - 3845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Matsumoto, A. J. Ellsmore, S. G. Cessna, P. S. Low, J. M. Pardo, R. A. Bressan, and P. M. Hasegawa An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae J. Biol. Chem., August 30, 2002; 277(36): 33075 - 33080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. V. Fedoroff Cross-Talk in Abscisic Acid Signaling Sci. Signal., July 9, 2002; 2002(140): re10 - re10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Briggs, Z. Li, and D. B. Sacks IQGAP1-mediated Stimulation of Transcriptional Co-activation by beta -Catenin Is Modulated by Calmodulin J. Biol. Chem., February 22, 2002; 277(9): 7453 - 7465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Pitt, R. D. Zuhlke, A. Hudmon, H. Schulman, H. Reuter, and R. W. Tsien Molecular Basis of Calmodulin Tethering and Ca2+-dependent Inactivation of L-type Ca2+ Channels J. Biol. Chem., August 10, 2001; 276(33): 30794 - 30802. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
