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Papers In Press, published online ahead of print September 8, 2000
J. Biol. Chem, 10.1074/jbc.M006375200
Submitted on July 18, 2000
Accepted on September 8, 2000

LMW-PTP controls the rate and the strenght of NIH-3T3 cells adhesion through its phosphorylation on tyrosine 131 or 132

Paola Chiarugi, Maria L. Taddei, Paolo Cirri, Doriana Talini, Francesca Buricchi, Guido Camici, Giampaolo Manao, Giovanni Raugei, and Giampietro Ramponi

Dipartimento di Scienze Biochimiche, Universita di Firenze, Firenze 50134

Corresponding Author: ramponi{at}scibio.unifi.it

The low molecular weight protein tyrosine phosphatase (LMW-PTP) is an enzyme involved in PDGF-induced mitogenesis and cytoskeleton rearrangement. Our previous results demonstrated that LMW-PTP is able to bind and dephosphorylate activated PDGF receptor, thus inhibiting cell proliferation. Recently we have shown that LMW-PTP is specifically phosphorylated by c-Src in a cytoskeleton-associated fraction in response to PDGF, and this phosphorylation increases about 20-fold LMW-PTP activity. LMW-PTP strongly influences cell adhesion, spreading and chemotaxis induced by PDGF stimulation, by regulating the phosphorylation level of p190Rho-GAP, a protein that is able to regulates Rho activity and hence cytoskeleton rearrangement. In the present study we investigate the physiological role of the two LMW-PTP tyrosine phosphorylation sites, using LMW-PTP mutants on tyrosine 131 or 132. We demonstrate that each tyrosine residue is involved in specific LMW-PTP functions. Both of them are phosphorylated during PDGF signalling. Phosphorylation on tyrosine 131 influences mitogenesis, dephosphorylating activated PDGF-R and cytoskeleton rearrangement, acting on p190RhoGAP. Phosphorylation on tyrosine 132 leads to an increase in the strength of cell substrate adhesion, downregulating matrix metallo-proteases expression, through the inhibition of Grb2/MAPK pathway. In conclusion, LMW-PTP tyrosine phosphorylation on both Tyr131 or Tyr132 cooperate to determine a faster and stronger adhesion to extracellular matrix, although these two events may diverge in timing and relative amount.


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