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Papers In Press, published online ahead of print August 17, 2000
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021
Corresponding Author: k-marians{at}ski.mskcc.org
There are two modes of DNA synthesis at a replication fork. The leading strand is synthesized in a continuous fashion in lengths that in Escherichia coli can be in excess of 2 Mb. On the other hand, the lagging strand is synthesized in relatively short stretches of 2 kb. Nevertheless, identical assemblies of the DNA polymerase III core tethered to the b sliding clamp account for both modes of DNA synthesis. Yet the same lagging-strand polymerase accounts for the synthesis of all Okazaki fragments at a replication fork, cycling repeatedly every one or two seconds from the 3¢-end of the just-completed fragment to the 3¢-end of the new primer. Several models have been invoked to account for the rapid cycling of a polymerase complex that can remain bound to the template for upward of 40 min. By using isolated replication protein-DNA template complexes, we have tested these models and show here that cycling of the lagging-strand polymerase can be triggered by either the action of primase binding to the replisome and synthesizing a primer or by collision of the lagging-strand polymerase with the 5´-end of the previous Okazaki fragment.
J. Biol. Chem, 10.1074/jbc.M006556200
Submitted on July 24, 2000
Accepted on August 16, 2000
Two distinct triggers for cycling of the lagging-strand polymerase at the replication fork
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