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Papers In Press, published online ahead of print September 29, 2000
J. Biol. Chem, 10.1074/jbc.M007070200
Submitted on August 4, 2000
Revised on September 28, 2000
Accepted on September 28, 2000
Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604
Corresponding Author: ralph.bash{at}asu.edu
Circular permutation analysis has detected fairly strong sites of intrinsic DNA bending on the promoter regions of the yeast GAL1-10 and GAL80 genes. These bends lie in functionally suggestive locations. On the promoter of the GAL1-10 structural genes, strong bends bracket nucleosome B, which lies between the UASG and the GAL1 TATA. These intrinsic bends could help position nucleosome B. Nucleosome B plus two other promoter nucleosomes protect the TATA and start site elements in the inactive state of expression but are completely disrupted (removed) when GAL1-10 expression is induced. The strongest intrinsic bend (~70°) lies at the downstream edge of nucleosome B. This places it ~30 bp upstream of the GAL1 TATA, a position that could allow it to be involved in GAL1 activation in several ways, including the recruitment of a yeast HMG protein that is required for the normally robust level of GAL1 expression in the induced state (Paull, T., Carey, M., and Johnson, R. (1996) Genes Dev 10, 2769-2781). On the regulatory gene GAL80, the single bend lies in the nonnucleosomal hypersensitive region, between a GAL80-specific far upstream promoter element and the more gene-proximal promoter elements. GAL80 promoter region nucleosomes contain no intrinsically bent DNA.
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