| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print October 24, 2000
Department of Pathology, Emory University, Atlanta, GA 30322
Corresponding Author: ono{at}bimcore.emory.edu
ADF/cofilin changes the twist of actin filaments by binding two longitudinally associated actin subunits. In the absence of an atomic model of the ADF/cofilin-F-actin complex, we have identified residues in ADF/cofilin that are essential for filament binding. Here, we have characterized the C-terminal tail of UNC-60B (a nematode ADF/cofilin isoform) as a novel determinant for its association with F-actin. Removal of the C-terminal isoleucine (Ile-152) by carboxypeptidase A or truncation by mutagenesis eliminated F-actin binding activity but strongly enhanced actin-depolymerizing activity. Replacement of Ile-152 by Ala had a similar but less marked effect: F-actin binding was weakened and depolymerizing activity slightly enhanced. Truncation of both Arg-151 and Ile-152 or replacement of Arg-151 with Ala also abolished F-actin binding and enhanced depolymerizing activity. Loss of F-actin binding in these mutants was accompanied by loss or greatly decreased severing activity. All of the variants of UNC-60B interacted with G-actin in an indistinguishable manner from wild-type. Cryoelectron microscopy showed that UNC-60B changed the twist of F-actin to a similar extent to vertebrate ADF/cofilins. Helical reconstruction and structural modeling of UNC-60B-F-actin complex reveal how the C-terminus of UNC-60B might be involved in one of the two actin-binding sites.
J. Biol. Chem, 10.1074/jbc.M007563200
Submitted on August 18, 2000
Revised on October 16, 2000
Accepted on October 24, 2000
The C-terminal Tail of UNC-60B (ADF/Cofilin) Is Critical for Maintaining Its Stable Association with F-actin and Is Implicated in The Second Actin-binding Site
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Ono, S. Yamashiro, and S. Ono Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad J. Cell Sci., August 15, 2008; 121(16): 2662 - 2670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamashiro, M. Gimona, and S. Ono UNC-87, a calponin-related protein in C. elegans, antagonizes ADF/cofilin-mediated actin filament dynamics J. Cell Sci., September 1, 2007; 120(17): 3022 - 3033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. O. Paavilainen, M. Hellman, E. Helfer, M. Bovellan, A. Annila, M.-F. Carlier, P. Permi, and P. Lappalainen Structural basis and evolutionary origin of actin filament capping by twinfilin PNAS, February 27, 2007; 104(9): 3113 - 3118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Dixon and P. J. Roy Muscle arm development in Caenorhabditis elegans Development, July 1, 2005; 132(13): 3079 - 3092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Quintero-Monzon, A. A. Rodal, B. Strokopytov, S. C. Almo, and B. L. Goode Structural and Functional Dissection of the Abp1 ADFH Actin-binding Domain Reveals Versatile In Vivo Adapter Functions Mol. Biol. Cell, July 1, 2005; 16(7): 3128 - 3139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, X. Liu, Z. Lou, X. Duan, H. Wu, Y. Liu, and Z. Rao Crystal structure of human coactosin-like protein at 1.9 A resolution Protein Sci., November 1, 2004; 13(11): 2845 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mohri, S. Vorobiev, A. A. Fedorov, S. C. Almo, and S. Ono Identification of Functional Residues on Caenorhabditis elegans Actin-interacting Protein 1 (UNC-78) for Disassembly of Actin Depolymerizing Factor/Cofilin-bound Actin Filaments J. Biol. Chem., July 23, 2004; 279(30): 31697 - 31707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ono and S. Ono Tropomyosin and Troponin Are Required for Ovarian Contraction in the Caenorhabditis elegans Reproductive System Mol. Biol. Cell, June 1, 2004; 15(6): 2782 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Pope, K. M. Zierler-Gould, R. Kuhne, A. G. Weeds, and L. J. Ball Solution Structure of Human Cofilin: ACTIN BINDING, pH SENSITIVITY, AND RELATIONSHIP TO ACTIN-DEPOLYMERIZING FACTOR J. Biol. Chem., February 6, 2004; 279(6): 4840 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Galkin, A. Orlova, M. S. VanLoock, A. Shvetsov, E. Reisler, and E. H. Egelman ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments J. Cell Biol., December 8, 2003; 163(5): 1057 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mohri and S. Ono Actin filament disassembling activity of Caenorhabditis elegans actin-interacting protein 1 (UNC-78) is dependent on filament binding by a specific ADF/cofilin isoform J. Cell Sci., October 15, 2003; 116(20): 4107 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. O. Paavilainen, M. C. Merckel, S. Falck, P. J. Ojala, E. Pohl, M. Wilmanns, and P. Lappalainen Structural Conservation between the Actin Monomer-binding Sites of Twinfilin and Actin-depolymerizing Factor (ADF)/Cofilin J. Biol. Chem., November 1, 2002; 277(45): 43089 - 43095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ono and K. Ono Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics J. Cell Biol., March 18, 2002; 156(6): 1065 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Palmgren, M. Vartiainen, and P. Lappalainen Twinfilin, a molecular mailman for actin monomers J. Cell Sci., January 3, 2002; 115(5): 881 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ono The Caenorhabditis elegans unc-78 Gene Encodes a Homologue of Actin-interacting Protein 1 Required for Organized Assembly of Muscle Actin Filaments J. Cell Biol., March 19, 2001; 152(6): 1313 - 1320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pfannstiel, M. Cyrklaff, A. Habermann, S. Stoeva, G. Griffiths, R. Shoeman, and H. Faulstich Human Cofilin Forms Oligomers Exhibiting Actin Bundling Activity J. Biol. Chem., December 21, 2001; 276(52): 49476 - 49484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Vartiainen, T. Mustonen, P. K. Mattila, P. J. Ojala, I. Thesleff, J. Partanen, and P. Lappalainen The Three Mouse Actin-depolymerizing Factor/Cofilins Evolved to Fulfill Cell-Type-specific Requirements for Actin Dynamics Mol. Biol. Cell, January 1, 2002; 13(1): 183 - 194. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
