| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print November 9, 2000
J. Biol. Chem, 10.1074/jbc.M007876200
Submitted on August 29, 2000
Revised on October 18, 2000
Accepted on November 8, 2000
Max-Planck-Institut for Molecular Genetics, Berlin D-14195
Corresponding Author: messer{at}molgen.mpg.de
Using combined PCR-gel retardation assay the preferred recognition sequence of the Streptomyces initiator protein DnaA was determined. The protein showed a preference towards DNA containing two E. coli-like DnaA boxes in head-to-head arrangement (consensus sequence TTATCCACA, whereas the consensus sequence of the DnaA boxes found in the Streptomyces oriC region is TTGTCCACA). In quantitative band shift experiments the kinetics of the Streptomyces DnaA - DnaA box interaction was characterized. The DnaA protein can form dimers while binding to a single DnaA box; dimer formation is mediated by the domain III of the protein and the dissociation constant of this process was between 35 and 115 nM. Streptomyces initiator protein DnaA interacts in a cooperative manner with DNA containing multiple binding sites. For the cooperativity effect, which seems to be independent of the distance separating the DnaA boxes, domain I (or I and II) is responsible. The cooperativity constant is moderate and is in the range 20-110.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Smulczyk-Krawczyszyn, D. Jakimowicz, B. Ruban-Osmialowska, A. Zawilak-Pawlik, J. Majka, K. Chater, and J. Zakrzewska-Czerwinska Cluster of DnaA Boxes Involved in Regulation of Streptomyces Chromosome Replication: from In Silico to In Vivo Studies. J. Bacteriol., September 1, 2006; 188(17): 6184 - 6194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Sibley, S. R. MacLellan, and T. Finan The Sinorhizobium meliloti chromosomal origin of replication Microbiology, February 1, 2006; 152(2): 443 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Capaldi and J. M. Berger Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus Nucleic Acids Res., September 9, 2004; 32(16): 4821 - 4832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujikawa, H. Kurumizaka, O. Nureki, T. Terada, M. Shirouzu, T. Katayama, and S. Yokoyama Structural basis of replication origin recognition by the DnaA protein Nucleic Acids Res., April 15, 2003; 31(8): 2077 - 2086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dziadek, M. Rajagopalan, T. Parish, N. Kurepina, R. Greendyke, B. N. Kreiswirth, and M. V. V. S. Madiraju Mutations in the CCGTTCACA DnaA Box of Mycobacterium tuberculosis oriC That Abolish Replication of oriC Plasmids Are Tolerated on the Chromosome J. Bacteriol., July 15, 2002; 184(14): 3848 - 3855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Carr and J. M. Kaguni Stoichiometry of DnaA and DnaB Protein in Initiation at the Escherichia coli Chromosomal Origin J. Biol. Chem., November 21, 2001; 276(48): 44919 - 44925. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
