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Papers In Press, published online ahead of print December 4, 2000
Institute of Molecular Biology & Tumor Research (IMT), Philipps University, Marburg 35033
Corresponding Author: mueller{at}imt.uni-marburg.de
Expression of the cdc25B gene is upregulated late during cell cycle progression (S/G2). We have cloned the murine cdc25B promoter to identify elements involved in transcriptional regulation. A detailed structure-function analysis led to the identification of several elements that are located upstream of a canonical Inr motif at the site of transcription initiation and are involved in transcriptional activation and regulation. Activation of the promoter is largely mediated by NF-Y and Sp1/3 interacting with one and four proximal binding sites, respectively. In addition, NF-Y plays an essential role in cell cycle regulation in conjunction with a repressor element (CCRR) located ~30 nucleotides upstream of the putative Inr element and overlapping a consensus TATA motif. The CCRR is unrelated to the previously described cell cycle-regulated repressor elements. Taken together, our observations suggest that expression of the cdc25B gene is controlled through a novel mechanism of cell cycle-regulated transcription.
J. Biol. Chem, 10.1074/jbc.M008696200
Submitted on September 22, 2000
Revised on November 2, 2000
Accepted on December 4, 2000
Cell cycle regulation of the murine cdc25B promoter: essential role for NF-Y and a proximal repressor element
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