| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print December 14, 2000
J. Biol. Chem, 10.1074/jbc.M009836200
Submitted on October 27, 2000
Revised on December 14, 2000
Accepted on December 14, 2000
Division of Human Immunology, Hanson Centre for Cancer research / Institute of Medical and Veterinary Science, Adelaide, South Australia 5000
Corresponding Author: peter.diamond{at}imvs.sa.gov.au
SUMMARY Cold shock domain (CSD) family members have been shown to play roles in either transcriptional activation or repression of many genes in various cell types. We have previously shown that CSD proteins dbpAv and dbpB (also known as YB-1) act to repress granulocyte-macrophage colony-stimulating factor (GM-CSF) transcription in human endothelial lung (HEL) fibroblasts via binding to single stranded DNA regions across the promoter. Here we show that the same CSD factors are involved in GM-CSF transcriptional activation in Jurkat T. Unlike the mechanisms of CSD repression in HEL fibroblasts, CSD mediated activation in Jurkat T cells is not mediated through DNA binding but presumably through protein:protein interactions via the C-terminus of the CSD protein with transcription factors such as RelA. We demonstrate that Jurkat T cells lack truncated CSD factor subtypes present in HEL fibroblasts, which raises the possibility that the cellular content of CSD proteins may determine their final role as activators or repressors of transcription.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. R.C. van Roeyen, F. Eitner, S. Martinkus, S. R. Thieltges, T. Ostendorf, D. Bokemeyer, B. Luscher, J. M. Luscher-Firzlaff, J. Floege, and P. R. Mertens Y-Box Protein 1 Mediates PDGF-B Effects in Mesangioproliferative Glomerular Disease J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2985 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Coles, P. Diamond, L. Lambrusco, J. Hunter, J. Burrows, M. A. Vadas, and G. J. Goodall A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y-box) proteins in normoxic fibroblasts Nucleic Acids Res., November 15, 2002; 30(22): 4845 - 4854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Norman, G. E. Lindahl, K. Shakib, A. En-Nia, E. Yilmaz, and P. R. Mertens The Y-box Binding Protein YB-1 Suppresses Collagen alpha 1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter J. Biol. Chem., August 3, 2001; 276(32): 29880 - 29890. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
