JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on April 13, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/16/12636    most recent
M010470200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sengupta, S. M.
Right arrow Articles by Bartholomew, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sengupta, S. M.
Right arrow Articles by Bartholomew, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 24, 2001
J. Biol. Chem, 10.1074/jbc.M010470200
Submitted on November 20, 2000
Revised on January 23, 2001
Accepted on January 24, 2001

The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling

Sarojini M. Sengupta, Michael VanKanegan, Jim Persinger, Colin Logie, Bradley R. Cairns, Craig L. Peterson, and Blaine Bartholomew

Biochemistry & Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-4413

Corresponding Author: bbartholomew{at}siumed.edu

Interactions of the yeast chromatin remodeling complexes SWI/SNF and RSC with nucleosomes were probed using site-specific DNA photoaffinity labeling. 5S rDNA was engineered with photoreactive nucleotides incorporated at different sites in DNA to scan for the subunits of SWI/SNF in close proximity to DNA when SWI/SNF is bound to the 5S nucleosome or to free 5S rDNA. The Swi2/Snf2 and Snf6 subunits of SWI/SNF were efficiently cross-linked at several positions in the nucleosome, whereas only Snf6 was efficiently cross-linked when SWI/SNF is bound to free DNA. DNA photoaffinity labeling of RSC showed that the Rsc4 subunit is in close proximity to nucleosomal DNA and not when RSC is bound to free DNA. After remodeling, the Swi2/Snf2 and Rsc4 subunits are no longer detected near the nucleosomal DNA and are evidently displaced from the surface of the nucleosome, indicating significant changes in SWI/SNF and RSC contacts with DNA after remodeling.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. Soutourina, V. Bordas-Le Floch, G. Gendrel, A. Flores, C. Ducrot, H. Dumay-Odelot, P. Soularue, F. Navarro, B. R. Cairns, O. Lefebvre, et al.
Rsc4 Connects the Chromatin Remodeler RSC to RNA Polymerases.
Mol. Cell. Biol., July 1, 2006; 26(13): 4920 - 4933.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. L. Smith and C. L. Peterson
A Conserved Swi2/Snf2 ATPase Motif Couples ATP Hydrolysis to Chromatin Remodeling
Mol. Cell. Biol., July 15, 2005; 25(14): 5880 - 5892.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. S. Kwon, C. Chen, and D. Wagner
WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of stem cell fate in Arabidopsis
Genes & Dev., April 15, 2005; 19(8): 992 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Wilsker, A. Patsialou, S. D. Zumbrun, S. Kim, Y. Chen, P. B. Dallas, and E. Moran
The DNA-binding properties of the ARID-containing subunits of yeast and mammalian SWI/SNF complexes
Nucleic Acids Res., February 24, 2004; 32(4): 1345 - 1353.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Aouida, N. Page, A. Leduc, M. Peter, and D. Ramotar
A Genome-Wide Screen in Saccharomyces cerevisiae Reveals Altered Transport As a Mechanism of Resistance to the Anticancer Drug Bleomycin
Cancer Res., February 1, 2004; 64(3): 1102 - 1109.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Gaillard, D. J. Fitzgerald, C. L. Smith, C. L. Peterson, T. J. Richmond, and F. Thoma
Chromatin Remodeling Activities Act on UV-damaged Nucleosomes and Modulate DNA Damage Accessibility to Photolyase
J. Biol. Chem., May 9, 2003; 278(20): 17655 - 17663.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J.-m. Hsu, J. Huang, P. B. Meluh, and B. C. Laurent
The Yeast RSC Chromatin-Remodeling Complex Is Required for Kinetochore Function in Chromosome Segregation
Mol. Cell. Biol., May 1, 2003; 23(9): 3202 - 3215.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Aoyagi, G. Narlikar, C. Zheng, S. Sif, R. E. Kingston, and J. J. Hayes
Nucleosome Remodeling by the Human SWI/SNF Complex Requires Transient Global Disruption of Histone-DNA Interactions
Mol. Cell. Biol., June 1, 2002; 22(11): 3653 - 3662.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. M. Fletcher, N. Xiao, G. Mautino, C. T. Baumann, R. Wolford, B. S. Warren, and G. L. Hager
ATP-Dependent Mobilization of the Glucocorticoid Receptor during Chromatin Remodeling
Mol. Cell. Biol., May 15, 2002; 22(10): 3255 - 3263.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. H. Ng, F. Robert, R. A. Young, and K. Struhl
Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex
Genes & Dev., April 1, 2002; 16(7): 806 - 819.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.